8 thoughts on “C64 – Shrestha

  1. overall super good job! you spoke very clearly and explained things super well. my question is at the start you were mentioning conjugation I believe but I was wondering what that is scientifically? I dont know much about that.

    1. Thank you! Conjugation is basically the mating period for T. thermophila. It’s a complicated process but essentially they exchange gametes with its mating partners and prepare for cell division so that they can later reproduce.

  2. You mentioned that T. thermophila cells have increased badcp expression during conjugation. Could you explain why T. thermophila experiences so much DNA damage during this process in comparison to other bacteria?

    1. I believe that it’s because T. thermophila have to introduce double-stranded breaks in the DNA so that they can properly undergo the exchange of genetic material and then repair the damage so that the exchanged DNA is incorporated into their owngenome

  3. Although your results were technically inconclusive, what would results that refute or support your hypothesis look like? And what would be the significance of these results?

    1. If Badcp was involved in DNA damage, we would see higher gene expression when DNA damage was induced, which would show up as a brighter +HU band. If it is not involved, gene expression would be unaffected by DNA damage so the UT and +HU bands would be equal brightness for Badcp primers. Either way, identifying whether genes are involved in DNA damage repair will contribute to our overall understanding of this super complicated process, which is significant for developing therapeutic interventions for diseases like cancer!

  4. Great presentation! I was curious, though, as to why there was more CDNA in the untreated samples and why exactly that would cause a lack of difference in expression of Rad51.

    1. Thanks so much! There are a couple possibilities for the unequal amounts of cDNA in our samples. The cDNA was synthesized by extracting RNA from the cells and then treating them with reverse transcriptase, which uses RNA as a template to reverse transcribe a cDNA sequence. It is difficult to control how well the reverse transcriptase works, so it is possible that the enzyme worked more efficiently in our UT samples than our +HU samples, producing more cDNA in the same amount of time from the same amount of RNA. The other possibility is that we made an error in preparing the PCR reactions and added more RNA to the UT sample than the +HU sample. Because there was likely a higher baseline expression of UT cDNA than expected, any increase in Rad51 expression due to DNA damage would show up as a smaller difference in brightness between the UT and +HU bands.

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