Hi, the gDNA was degraded in all lanes of the PCR for everyone in my lab section, so it was likely a handling or storage error before we ever touched it. Some possibilities included too hot or too cold temperatures for storage or improper lid closure leading to evaporation.
Much like how a PCR was run before and after DNA damage to determine Wd40’s involvement in the DNA damage repair pathway, other PCRs with different conditions could test involvement in other pathways. For example, a PCR run with a control and heat shock or control and starvation conditions could test Wd40’s involvement in heat shock or starvation, respectively.
curesymposium.org 
What caused the genes to become degraded?
LikeLike
Hi, the gDNA was degraded in all lanes of the PCR for everyone in my lab section, so it was likely a handling or storage error before we ever touched it. Some possibilities included too hot or too cold temperatures for storage or improper lid closure leading to evaporation.
LikeLike
Hi! what other experiments could be performed to discover the actual function of the WD40 gene?
LikeLike
Much like how a PCR was run before and after DNA damage to determine Wd40’s involvement in the DNA damage repair pathway, other PCRs with different conditions could test involvement in other pathways. For example, a PCR run with a control and heat shock or control and starvation conditions could test Wd40’s involvement in heat shock or starvation, respectively.
LikeLike