qPCR would provide more accurate, quantitative data to confirm that Rsp1 gene expression increases after DNA damage induction. A western blot would confirm whether Rsp1 protein concentration also increases after DNA damage is induced.
DNA damage does not necessarily have a beneficial effect for cells, but it is a normal occurrence. Something as simple as exposure to sunlight can lead to dna damage. Most of the time, this damage can be fixed through dna damage repair pathways in our bodies, but other times repair fails to fix the damage and that is when abnormal cell growth can occur.
This is just the first step in find new treatment methods for DNA damage related diseases. Concluding that Rsp1 and other sRNA-related genes/proteins are involved in the DNA damage repair pathway now allows research to move into better understand the mechanism with which these proteins and sRNAs help with the process. Future treatments could then target these mechanisms and thereby fix the problem at the very source.
This is just the first step in developing new treatments for DNA damage related diseases. This now opens the door to conducting more specific research into the exact mechanisms with which Rsp1 and Rsp1-related sRNAs are involved in the DNA damage repair pathway. New treatment would then likely target these mechanisms to fix the issue at the source.
curesymposium.org 
Well done with your poster and presentation! How would qPCR and preforming a western blot allow you to further confirm your hypothesis?
LikeLike
qPCR would provide more accurate, quantitative data to confirm that Rsp1 gene expression increases after DNA damage induction. A western blot would confirm whether Rsp1 protein concentration also increases after DNA damage is induced.
LikeLike
Hi Renee, great presentation! How specifically can DNA-damage cause diseases yet benefit them at the same time?
LikeLike
DNA damage does not necessarily have a beneficial effect for cells, but it is a normal occurrence. Something as simple as exposure to sunlight can lead to dna damage. Most of the time, this damage can be fixed through dna damage repair pathways in our bodies, but other times repair fails to fix the damage and that is when abnormal cell growth can occur.
LikeLike
Awesome job! If this solution was fully developed how would it be administered to patients with neurodegenerative disorders or cancers?
LikeLike
This is just the first step in find new treatment methods for DNA damage related diseases. Concluding that Rsp1 and other sRNA-related genes/proteins are involved in the DNA damage repair pathway now allows research to move into better understand the mechanism with which these proteins and sRNAs help with the process. Future treatments could then target these mechanisms and thereby fix the problem at the very source.
LikeLike
Great presentation. How could this be used in future treatments?
LikeLike
This is just the first step in developing new treatments for DNA damage related diseases. This now opens the door to conducting more specific research into the exact mechanisms with which Rsp1 and Rsp1-related sRNAs are involved in the DNA damage repair pathway. New treatment would then likely target these mechanisms to fix the issue at the source.
LikeLike
Very nice presentation! Do you think this kind of testing would also work in prokaryotes?
LikeLike