Hi Jocelyn! We kept having to redo our gels because the PCR tubes were dehydrating so we weren’t getting complete results for while. It was very time consuming but eventually we were able to get all our PCR tubes to work!
Really good presentation, you did a great job not reading directly off the poster. What was the benefit of designing JEG if RPPO and DND already anneal to WD40?
Hi Daniel! Designing JEG was a project we did within the class to learn how to use the programs Primer3Plus and BLAST which are very important for cell bio research. BLAST is a national database that can rapidly compare almost any query sequence you want which is a way to compare genomes. We learned how to use it and learned how to design a primer which are both essential research skills. As a plus, if our primer worked we could use it in our research project for the class. We couldn’t tell if JEG worked because of the PCR problem I described above so we used DND which the lab provided just on the chance that JEG didn’t work in our final gel we ran. RPP0 was the control, so like any research experiment this was used to verify the results we were seeing as true and not just due to chance.
curesymposium.org 
Really interesting presentation, I feel like I’ve learned a lot! What would you say was the most challenging part of your experimental process?
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Hi Jocelyn! We kept having to redo our gels because the PCR tubes were dehydrating so we weren’t getting complete results for while. It was very time consuming but eventually we were able to get all our PCR tubes to work!
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Really good presentation, you did a great job not reading directly off the poster. What was the benefit of designing JEG if RPPO and DND already anneal to WD40?
LikeLike
Hi Daniel! Designing JEG was a project we did within the class to learn how to use the programs Primer3Plus and BLAST which are very important for cell bio research. BLAST is a national database that can rapidly compare almost any query sequence you want which is a way to compare genomes. We learned how to use it and learned how to design a primer which are both essential research skills. As a plus, if our primer worked we could use it in our research project for the class. We couldn’t tell if JEG worked because of the PCR problem I described above so we used DND which the lab provided just on the chance that JEG didn’t work in our final gel we ran. RPP0 was the control, so like any research experiment this was used to verify the results we were seeing as true and not just due to chance.
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