Hi Noelle. So in this experiment, we didn’t increase the expression of Hypedc3. Rather, we measured the gene expression in normal T. thermophila cells and DNA damaged T. thermophila. Based on our results we were able to conclude that DNA damaged cells showed higher expression of the Hypedc3 gene, meaning that the gene gets up-regulated during DNA damage. This means that we cannot decrease the expression of Hypedc3. However, we recommend that future Cas9 knockout experiments be conducted. The experiment, would include silencing the Hypedc3 gene and seeing what happens after DNA damage.
From our experiment all we can conclude is that the Hypedc3 gene becomes up-regulated following DNA damage. This lead us to conclude that the Hypedc3 is likely involved in the DNA damage repair pathway, however, we are not sure what function it specifically it serves. With the knock out experiments the Hypedc3 gene would be silenced, allowing to see if it is truly part of the pathway. Additionally, this would allow us to see what the function of the gene is by seeing if silencing the gene limits the cell’s ability to repair its DNA. For example, if we find that when the gene is silence a certain step of the repair mechanism does not occur, then we can narrow down where the gene might be involved. We expect that these experiments will show us that the Hypedc3 is definitely involved in the repair pathway. However, currently I am unable to make any guesses to its function. Hypedc3 has no known domains or homologs so it is really impossible to compare it with anything else that is known.
curesymposium.org 
After this experiment, what would you expect to happen if you were to decrease the expression of Hypedc3 rather than increasing it?
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Hi Noelle. So in this experiment, we didn’t increase the expression of Hypedc3. Rather, we measured the gene expression in normal T. thermophila cells and DNA damaged T. thermophila. Based on our results we were able to conclude that DNA damaged cells showed higher expression of the Hypedc3 gene, meaning that the gene gets up-regulated during DNA damage. This means that we cannot decrease the expression of Hypedc3. However, we recommend that future Cas9 knockout experiments be conducted. The experiment, would include silencing the Hypedc3 gene and seeing what happens after DNA damage.
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What might you expect the results to conclude from the CRISPR knockout experiment for the functions of Hypedc3?
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From our experiment all we can conclude is that the Hypedc3 gene becomes up-regulated following DNA damage. This lead us to conclude that the Hypedc3 is likely involved in the DNA damage repair pathway, however, we are not sure what function it specifically it serves. With the knock out experiments the Hypedc3 gene would be silenced, allowing to see if it is truly part of the pathway. Additionally, this would allow us to see what the function of the gene is by seeing if silencing the gene limits the cell’s ability to repair its DNA. For example, if we find that when the gene is silence a certain step of the repair mechanism does not occur, then we can narrow down where the gene might be involved. We expect that these experiments will show us that the Hypedc3 is definitely involved in the repair pathway. However, currently I am unable to make any guesses to its function. Hypedc3 has no known domains or homologs so it is really impossible to compare it with anything else that is known.
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