17 thoughts on “D113 – Conant

  1. Great poster! What were your positive/negative controls and why did you choose those?

    1. I used DMSO as the negative control because it is a solvent with no antibacterial properties and it can dissolve other contaminants that may have gotten into a well. I used ampicillin as the positive control because it is a known antibiotic for Salmonella Typhimurium.

  2. How did you determine the concentrations you would use for the dose curve evaluation?

    1. We struggled to find the primary concentration of the Vidatox so we just followed a 1:10 dilution of the stock solution for our five concentrations.

    1. We studied salmonella typhimurium because it was widely available to us and because it is a strain of Salmonella that is much safer than Salmonella Typhi. This allowed us to get good research done on a safer bacteria that could be applied to a more dangerous bacteria.

  3. Good job! What do you think made the absorbance go up as concentration got lower (from 10% and lower) if the compound was a growth promoter?

    1. In our first trial, with a concentration of 10%, we believe that although Vidatox appeared clear to the human eye, at such a high concentration it could have affected the spectrophotometer and skewed the data for that concentration.

  4. Hi Gus very nice job on your presentation! I noticed you mentioned a bactericidal/static experiment in your methods section. Did you end up running this experiment seeing as Vidatox is a growth promoter? What results would you have expected to see if you didn’t run it?

    1. We did run a bacteriostatic/cidal test and we found no significant results from the test. Before we ran any trials we expected Vidatox to be bacteriostatic.

  5. Good Job! I noticed on values below 10% concentration it looked as though growth was increasing as concentration goes down. This seems like if the compound promotes growth for bacteria, then less of it should lead to less growth. Why do you think absorbency increased despite smaller concentrations?

    1. In our first trial, with a concentration of 10%, we believe that although Vidatox appeared clear to the human eye, at such a high concentration it could have affected the spectrophotometer and skewed the data for that concentration.

  6. Good job! If the compound was a growth promoter, why do you think decreasing concentrations to trace amounts led to increased growth?

    1. The growth shown would most likely be due to human error while pipetting, it was a very insignificant change in the amount of growth.

  7. Hey Gus, great presentation. How was a ‘hit’ determined, or how did did you know whether a drug was a hit or not?

    1. A “hit” was determined if the absorbance of the Vidatox was outside of two standard deviations from the average of our negative control.

  8. Great job! My one question is why the evidence of anti-cancer properties for this compound made you believe it would be useful as an antibiotic?

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