The positive control did not have much variance because of the already known results and concentration of it, even though CHL and AMP were both used for positive controls they were consistent. The DMSO may have also been old when we used it so that could have been a major factor as to why it varied.
If there was a positive hit we would’ve continued with the dilution series and do a bacteriocidal/static test with a new plate observing it over 24 hours to see the growth of the bacteria.
Why do you think the positive control treatment did not appear to appreciably increase its variance in your results, while the negative control did?
The positive control did not have much variance because of the already known results and concentration of it, even though CHL and AMP were both used for positive controls they were consistent. The DMSO may have also been old when we used it so that could have been a major factor as to why it varied.
If there were to have been a positive ‘hit’ on your test, what would you have done next?
If there was a positive hit we would’ve continued with the dilution series and do a bacteriocidal/static test with a new plate observing it over 24 hours to see the growth of the bacteria.