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8 thoughts on “D24 – Huo”
What is the point of using the plates and how does plate research translate to real life implications? i.e. what do the plates mimic if anything at all?
The function of these plates is to allow us to more directly see the effect of the compound on Salmonella Typhimurium under the condition of sufficient nutrients. This at least shows that this substance cannot prevent the growth of Salmonella Typhimurium in normal human cells. So we need to extract the single compounds in the substance separately for further experiments.
What do you think caused your Chaulmoogric oil extraction to not be able to kill the bacteria?
Chaulmoogric oil extraction is likely to contain nutrient compounds that can stimulate the growth of Salmonella Typhimurium. In this case, even if certain components in Chaulmoogric oil extraction can kill Salmonella Typhimurium, it is difficult for us to observe from the results.
Can you explain the method of ethanol extraction?
we put 10 mL of 70% stock solution ethanol (EtOH) in a conical tube with 10 uL of our compound, then vortexed the tube for 5 seconds every 5 minutes over a span of 30 minutes, which resulted in an oil layer atop our solution.
What are some of the uncontrollable variations that you avoid by completing 10 times?
as we did do that yet, so i don’t really know what we can avoid. However, I expected to avoid the growth of bacteria by long time preparation of the tests.