10 thoughts on “D26 – Enlow

  1. How would future replicates differ in testing SPIONs against Drosophila cells, also what was the cell types that were tested as I lost that in the intro?

    1. Sorry you missed that! We did not use a specific cell line, but rather the entire Drosophila melanogaster larva. The larvae acted as miniature tumors due to their high prolificacy and moderate cell differentiation (similar to a tumor).

      I am not sure what you mean by ‘replicates’ could you elaborate further?

  2. So what exactly was your first step in the process and why since you only completed two trials, how many were you supposed to do and how would that change your overall results?

    1. This was the first step technically, but I may be misunderstanding your question (apologies if so). We technically could have only run one trial if we had had 50+ pupal cases/vial (instead we averaged <5). More trials should lead to more data should lead to more certainty in the accuracy of our results

  3. If you were to perform this experiment again and had the proper amount of time with the SPIONs, how many additional times would you run your experiment?

    1. 1,000,000!!

      Not really. It would depend on how good our data seemed to be. If we had minimum 50 pupal cases per vial, I would feel confident to move onto either different concentrations, MFH, or completely scrap the project, depending on the results.

      We only had ~80 pupal cases total and we were hoping for >1,200

  4. Why do you think that there wasn’t much of a difference between your raw and edited data and why do you think that a trendline was not able to be identified?

    1. We are not sure if A) the SPIONs were extremely cytotoxic, thus killing the vast majority of larvae prior to pupating B) simply not many larvae were added to the vials and we did not have enough data points (we did not place the larvae into the vials personally) C) the larvae were attacked by a bacteria (apparently some experiments had this problem) D) all the above.

      In the end, it was a major problem with limited data. We only had ~80 pupal cases between all the vials and we were hoping for ~1,200.

  5. If you were to design a follow-up experiment using the same tumor model, what changes to your current procedure would you make?

    1. Most importantly, I would personally count the number of D. melanogaster larvae that were added to each test vial. From there I would like to use coated SPIONs, and utilize a fibrillating magnet to hopefully induce MFH

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