Thanks Fiona! At this point no, this experiment proved that the DNA replication attributes of decitabine were enough to kill Salmonella but it didn’t do anything to prove if it would have a similar effect on the cells in the body. As you can imagine, if it killed the cells bodies at the same rate as salmonella that would be an issue.
How did the results from the dose response curve experiment show that the compound acted more like a drug than a poison? Like what would the results of a compound that acted more like a poison look like?
If there was a large jump in effectiveness between one dilution series to another that would indicate a poison. Since our dilution series shows a more gradual loss of efficacy its easier to titrate the drug which in turn reduces the risk of unwanted side effects.
If our dose response curve was very steep that would be indicative of a poison. The steep curve would make it really hard to dose the drug effectively.
This came from the rate of decreased effectiveness of our compound. You can see that in our dose response curve the efficacy of the drug is lost gradually. If there was a sharper drop off in efficacy it would indicate a poison.
If you were able to complete the goals you stated in your future directions, how would your project’s results contribute to the broader research being done surrounding antibiotic resistance?
If we could prove that this was a viable drug option it would allow for another “angle”, so to speak, to attack antibiotic resistant bacteria from. It wouldn’t be solving the issue, but rather buying more time to look into a longer term solution.
A poison wouldn’t leave enough room for titration during treatment. Essentially the way I understand it, the steep curve of a poison would make the effective dose and the toxic dose to narrow to be beneficial. I won’t say there is never going to be a poison that can be used as an antibiotic but it would be far more difficult to justify its use with all the potential dangers.
If you were to complete your goals that you stated in your future directions, how would your results contribute to the broader research being done about antibiotic resistance?
If we could prove that this was a viable drug option it would allow for another “angle”, so to speak, to attack antibiotic resistant bacteria from. It wouldn’t be solving the issue, but rather buying more time to look into a longer term solution.
If you were to complete the goals you stated in your future directions, how would your project contribute to the broader research being done with antibiotic resistance?
If we could prove that this was a viable drug option it would allow for another “angle”, so to speak, to attack antibiotic resistant bacteria from. It wouldn’t be solving the issue, but rather buying more time to look into a longer term solution.
This was a great presentation! Would you currently feel safe using something like Decitabine if you had the opportunity/needed to?
Thanks Fiona! At this point no, this experiment proved that the DNA replication attributes of decitabine were enough to kill Salmonella but it didn’t do anything to prove if it would have a similar effect on the cells in the body. As you can imagine, if it killed the cells bodies at the same rate as salmonella that would be an issue.
How did the results from the dose response curve experiment show that the compound acted more like a drug than a poison? Like what would the results of a compound that acted more like a poison look like?
If there was a large jump in effectiveness between one dilution series to another that would indicate a poison. Since our dilution series shows a more gradual loss of efficacy its easier to titrate the drug which in turn reduces the risk of unwanted side effects.
You’ve determine decitabine to be a drug and not a poison, what would classify a compound as a poison?
If our dose response curve was very steep that would be indicative of a poison. The steep curve would make it really hard to dose the drug effectively.
How do you know the difference between a poisonous compound versus a nontoxic or medicinal compound?
This came from the rate of decreased effectiveness of our compound. You can see that in our dose response curve the efficacy of the drug is lost gradually. If there was a sharper drop off in efficacy it would indicate a poison.
If you were able to complete the goals you stated in your future directions, how would your project’s results contribute to the broader research being done surrounding antibiotic resistance?
If we could prove that this was a viable drug option it would allow for another “angle”, so to speak, to attack antibiotic resistant bacteria from. It wouldn’t be solving the issue, but rather buying more time to look into a longer term solution.
How would a poison affect the organism differently? Can a substance be a drug AND a poison or is that not possible?
A poison wouldn’t leave enough room for titration during treatment. Essentially the way I understand it, the steep curve of a poison would make the effective dose and the toxic dose to narrow to be beneficial. I won’t say there is never going to be a poison that can be used as an antibiotic but it would be far more difficult to justify its use with all the potential dangers.
If you were to complete your goals that you stated in your future directions, how would your results contribute to the broader research being done about antibiotic resistance?
If we could prove that this was a viable drug option it would allow for another “angle”, so to speak, to attack antibiotic resistant bacteria from. It wouldn’t be solving the issue, but rather buying more time to look into a longer term solution.
If you were to complete the goals you stated in your future directions, how would your project contribute to the broader research being done with antibiotic resistance?
If we could prove that this was a viable drug option it would allow for another “angle”, so to speak, to attack antibiotic resistant bacteria from. It wouldn’t be solving the issue, but rather buying more time to look into a longer term solution.