Additionally, you talked about not having enough time for your project. What would you have done with more time? Do you think this would have affected your inconclusive conclusion on Elderberry antibiotic against Salmonella?
The ability to have a wider range of doses and spend more time honing exactly what experimental tactic we wanted to use would have given us slightly more confidence in our results, but overall we feel that the results were generally conclusive in determining that elderberry was an ineffective antibiotic compound at the doses we tested.
Hi Ankit! In fact, a higher absorbance is a sign of lower antibiotic activity! The lower the absorbance, the fewer bacteria there were to block the waves of the spectrophotometer, which means that bacterial growth was blocked or killed.
Hi Neko, great presentation! Also excellent last name. Love your focus on traditional medicine as well. What compounds from elderberry were hypothesized to be antimicrobial? and how might those function to kill bacteria or promote recovery?
Hi Conner (twinning!),
The high number of acidic polyphenols found in elderberry compared to other berries are the most prominent bioactive compounds, and polyphenols ae known to act as antimicrobial agents. Thanks for the question.
Why did you consider the most bioactive from the graph 5ug/mL? It looks like DMSO is higher than that in terms of Average Absorbance
Additionally, you talked about not having enough time for your project. What would you have done with more time? Do you think this would have affected your inconclusive conclusion on Elderberry antibiotic against Salmonella?
The ability to have a wider range of doses and spend more time honing exactly what experimental tactic we wanted to use would have given us slightly more confidence in our results, but overall we feel that the results were generally conclusive in determining that elderberry was an ineffective antibiotic compound at the doses we tested.
Hi Ankit! In fact, a higher absorbance is a sign of lower antibiotic activity! The lower the absorbance, the fewer bacteria there were to block the waves of the spectrophotometer, which means that bacterial growth was blocked or killed.
What results did your agar plates show?
Hi! In fact, all of our experiments were run on 96 well plates, and none of our experimentation involved agar plates.
Hi Neko, great presentation! Also excellent last name. Love your focus on traditional medicine as well. What compounds from elderberry were hypothesized to be antimicrobial? and how might those function to kill bacteria or promote recovery?
Hi Conner (twinning!),
The high number of acidic polyphenols found in elderberry compared to other berries are the most prominent bioactive compounds, and polyphenols ae known to act as antimicrobial agents. Thanks for the question.