6 thoughts on “D2b – Woodard

    1. Dante, Salmonella Typhimurium gives results very quickly compared to other bacteria. So it is not necessarily that the Salmonella it self is replicable but that the experiments done with are easily replicated and since these experiments are being done by different groups or people, we want them to be as easily replicable as possible so those that come after us will have success just as we did.

    1. Emma, Salmonella was not the positive control in this experiment. The positive control is the Ampicillin (APM) which is the best antibiotic on the market for treating Salmonella Typhimurium as of now. We made this our positive control because we were able to compare the absorbance in the wells with AMP in it to the wells that had the Methylglyoxal dilutions in them. Our negative control was the water because it was the mode of transportation that has been proven to not interact with Salmonella Typhimurium. This allowed us to see what the growth of the Salmonella would have been in the human body without interaction of anything at all.

    1. We chose the Manuka Honey to begin with and then based on the results that previous semesters have provided, we decided to isolate the Methylglyoxal for this semester specifically. The sugar that is found in normal “household” honey has been found to promote the growth of Salmonella Typhimurium. Manuka Honey still has these sugars in it but since it also has the MGO in it, the effects are not as serious as it would be with other honey.

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