6 thoughts on “D43 – Quirk

    1. Hi! Japanese Knotweed has our compound resveratrol, and also a chemical called emodin, so this complicates the results since we do not know if it is resveratrol that is promoting growth/death or if it is the emodin.

  1. What exactly is a mueller hinton diffusion test?? Also if you were to do the lab again would you have been able to increase the dose? If the dose were increased would that jump then allow for you to see results of bacteria being killed?

    1. Hi! A Mueller Hinton diffusion test is a test that looks at the bacterial growth on an agar plate when a compound interacts with it. You place a thin layer of bacteria over your whole agar plate and then place your positive control, negative control, and compound into three different holes in the agar plate. The plate is placed in an incubator to allow it to grow over 24-48 hours. The compounds will diffuse into the agar and interact with the bacteria overtime. If the compound has antibiotic properties, there will an area around the hole that had the compound, called the area of inhibition, that the bacteria did not grow.
      I think we could increase the dose concentration and that might be better at killing bacteria. The issue of that would be that the compound would get too dark to get good readings on the spectrophotometer, but there may be better results in the Mueller Hinton test.

  2. How would the different color of the compound positively or negatively change the results?

    1. The dark color of our compound, Resveratrol, negatively impacted our results because the spectrophotometer gives results based off of the absorbance. The higher the absorbance, the more bacteria that were in the wells, but the color also increases the absorbance. Since the controls were clear or difficult to get the same color, we could not compare the controls to the resveratrol absorbance. That’s why a future direction would be getting a colorless compound to work with.

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