How do you suspect the graph would change if the compound that was 100% EGCg? Which of the other compounds making up the additional 50% do you believe made the biggest negative impact on the data?
It’s difficult to determine which ingredient had the biggest negative effect on the potential antibiotic properties of EGCG in the capsule. However, it’s possible that the presence of other catechins in the Green Tea Extract could have influenced the results. While EGCG is the most abundant catechin in green tea, other catechins such are also present in smaller amounts. These may have different antimicrobial properties and could have interacted with EGCG in the capsule, potentially reducing its effectiveness against Salmonella. In addition, the capsule contained other ingredients such as hypromellose (cellulose capsule), microcrystalline cellulose, stearic acid (vegetable source), and silicon dioxide. These ingredients may have influenced the absorption, of EGCG, potentially reducing its antimicrobial activity against Salmonella.
Overall, the interaction of multiple factors could have contributed to the results obtained, and skewed our results in this study.
Additionally, While EGCG has been studied for its potential health benefits, including antioxidant, anti-inflammatory, and anticancer properties, there is limited evidence to suggest that it has direct antibiotic effects. Therefore, we would need to conduct another study and see directly what the graph would look like, and if it would prove our initial hypothesis, of being a compound with antibiotic properties.
Additionally, in regard to the graph, while EGCG has been studied for its potential health benefits, including antioxidant, anti-inflammatory, and anticancer properties, there is limited evidence to suggest that it has direct antibiotic effects. Therefore, I believe we would need to conduct another study in order to truly know what the graph would look like, and whether or not it would continue the trend of some other promising studies that showcase its ability to act as an antibiotic, as stated in our hypothesis.
Hi your poster and presentation was very concise and well spoken. Why did the dose response curve experiment for with vs without dye have skewed results? (pertaining to what you discussed around 2:33)
Hi Aeva, Thank you. Yes, when using the dye and putting our 96 well plate through the spectrophotometer, we ran into an issue with a failure of our negative control, DMSO. We attempted to run a plate several times, but consistently ran into the failure of our negative control. If the negative control, in this case DMSO, fails in a spectrophotometer experiment, it could be due to a number of reasons including, Impurities in the DMSO: if the DMSO used as the negative control was not pure or contained impurities, it could affect the results of the experiment. Impurities in the solvent can cause interference or absorbance at the same wavelength as the substance being measured, leading to a false positive or false negative result.
Or, another reason could be contamination during preparation. If the DMSO was contaminated during preparation or handling, it could affect the results of the experiment. Contamination could occur if the DMSO was not stored properly, if the equipment used was not clean, or if there was cross-contamination from other samples. Additionally, If there were technical errors during the experiment, such as incorrect calibration of the spectrophotometer or incorrect measurement of the DMSO, it could lead to a failure of the negative control. However, after multiple attempts and careful care, we decided to remove the dye as it could have potentially played a role in contaminating, technical issues, impurity, etc, and we wanted to eliminate the risk of such potentially coming from the dye. When we did, our negative control (DMSO) immediately worked.
The methods for using pure EGCG in a spectrophotometer experiment would be similar to those used with EGCG in a capsule, with a few differences:
With pure EGCG, it would be important to prepare a stock solution of the compound in an appropriate solvent, such as water or ethanol, and dilute it to the desired concentration for the experiment. The concentration and purity of the EGCG should be measured and monitored to ensure accurate results. Depending on the solvent used to prepare the EGCG solution, a different solvent may need to be used as the negative control in the spectrophotometer experiment. For example, if EGCG is dissolved in ethanol, the negative control could be ethanol alone. The absorption spectrum of pure EGCG may differ slightly from that of EGCG in a capsule, due to potential interactions with other compounds in the capsule. Therefore, it would be important to measure the absorption spectrum of the pure EGCG solution to ensure accurate measurements. With pure EGCG, it may be possible to use a wider concentration range in the spectrophotometer experiment, as the concentration of EGCG in the solution can be precisely controlled. This could allow for a more detailed analysis of the relationship between concentration and absorbance. Overall, the methods for using pure EGCG in a spectrophotometer experiment would be similar to those used with EGCG in a capsule, but with a greater emphasis on precise preparation, accurate measurement of concentration and purity, and careful selection of solvent and negative control.
How do you suspect the graph would change if the compound that was 100% EGCg? Which of the other compounds making up the additional 50% do you believe made the biggest negative impact on the data?
It’s difficult to determine which ingredient had the biggest negative effect on the potential antibiotic properties of EGCG in the capsule. However, it’s possible that the presence of other catechins in the Green Tea Extract could have influenced the results. While EGCG is the most abundant catechin in green tea, other catechins such are also present in smaller amounts. These may have different antimicrobial properties and could have interacted with EGCG in the capsule, potentially reducing its effectiveness against Salmonella. In addition, the capsule contained other ingredients such as hypromellose (cellulose capsule), microcrystalline cellulose, stearic acid (vegetable source), and silicon dioxide. These ingredients may have influenced the absorption, of EGCG, potentially reducing its antimicrobial activity against Salmonella.
Overall, the interaction of multiple factors could have contributed to the results obtained, and skewed our results in this study.
Additionally, While EGCG has been studied for its potential health benefits, including antioxidant, anti-inflammatory, and anticancer properties, there is limited evidence to suggest that it has direct antibiotic effects. Therefore, we would need to conduct another study and see directly what the graph would look like, and if it would prove our initial hypothesis, of being a compound with antibiotic properties.
Additionally, in regard to the graph, while EGCG has been studied for its potential health benefits, including antioxidant, anti-inflammatory, and anticancer properties, there is limited evidence to suggest that it has direct antibiotic effects. Therefore, I believe we would need to conduct another study in order to truly know what the graph would look like, and whether or not it would continue the trend of some other promising studies that showcase its ability to act as an antibiotic, as stated in our hypothesis.
Hi your poster and presentation was very concise and well spoken. Why did the dose response curve experiment for with vs without dye have skewed results? (pertaining to what you discussed around 2:33)
Hi Aeva, Thank you. Yes, when using the dye and putting our 96 well plate through the spectrophotometer, we ran into an issue with a failure of our negative control, DMSO. We attempted to run a plate several times, but consistently ran into the failure of our negative control. If the negative control, in this case DMSO, fails in a spectrophotometer experiment, it could be due to a number of reasons including, Impurities in the DMSO: if the DMSO used as the negative control was not pure or contained impurities, it could affect the results of the experiment. Impurities in the solvent can cause interference or absorbance at the same wavelength as the substance being measured, leading to a false positive or false negative result.
Or, another reason could be contamination during preparation. If the DMSO was contaminated during preparation or handling, it could affect the results of the experiment. Contamination could occur if the DMSO was not stored properly, if the equipment used was not clean, or if there was cross-contamination from other samples. Additionally, If there were technical errors during the experiment, such as incorrect calibration of the spectrophotometer or incorrect measurement of the DMSO, it could lead to a failure of the negative control. However, after multiple attempts and careful care, we decided to remove the dye as it could have potentially played a role in contaminating, technical issues, impurity, etc, and we wanted to eliminate the risk of such potentially coming from the dye. When we did, our negative control (DMSO) immediately worked.
Would any of your methods change upon using the pure EGCg
The methods for using pure EGCG in a spectrophotometer experiment would be similar to those used with EGCG in a capsule, with a few differences:
With pure EGCG, it would be important to prepare a stock solution of the compound in an appropriate solvent, such as water or ethanol, and dilute it to the desired concentration for the experiment. The concentration and purity of the EGCG should be measured and monitored to ensure accurate results. Depending on the solvent used to prepare the EGCG solution, a different solvent may need to be used as the negative control in the spectrophotometer experiment. For example, if EGCG is dissolved in ethanol, the negative control could be ethanol alone. The absorption spectrum of pure EGCG may differ slightly from that of EGCG in a capsule, due to potential interactions with other compounds in the capsule. Therefore, it would be important to measure the absorption spectrum of the pure EGCG solution to ensure accurate measurements. With pure EGCG, it may be possible to use a wider concentration range in the spectrophotometer experiment, as the concentration of EGCG in the solution can be precisely controlled. This could allow for a more detailed analysis of the relationship between concentration and absorbance. Overall, the methods for using pure EGCG in a spectrophotometer experiment would be similar to those used with EGCG in a capsule, but with a greater emphasis on precise preparation, accurate measurement of concentration and purity, and careful selection of solvent and negative control.