From my research, I concluded that betulin has a lot of potential and with a lot of more testing could ultimately make it to further trials. My group was able to get a statistical hit and stop the growth of S. Typhimurium, so I can confidently say that it’s a bacteriostatic antibiotic!
I would say the fact that betulin is known to have insoluble traits definitely had a huge impact on where errors came into the picture. It was for sure difficult to get this compound at a physiologically relevant concentration at which it could dissolve in DMSO, and my group and I weren’t able to get this concentration right until the end of our research sadly.
Since betulin did come in powder form, I would think a limitation to using it as an antibiotic would be transforming it into the perfect concentration and stock solution that best stops the growth of Salmonella Typhimurium. There needs to be a lot of more testing done, especially in mice and eventually humans to see how this compound and the subject reacts!
How has this research that you conducted overall contribute to your general understanding of Betulin and the effects that it has on S. Typhimurium.
From my research, I concluded that betulin has a lot of potential and with a lot of more testing could ultimately make it to further trials. My group was able to get a statistical hit and stop the growth of S. Typhimurium, so I can confidently say that it’s a bacteriostatic antibiotic!
You noted some causes of error, which do you think had the largest impact?
I would say the fact that betulin is known to have insoluble traits definitely had a huge impact on where errors came into the picture. It was for sure difficult to get this compound at a physiologically relevant concentration at which it could dissolve in DMSO, and my group and I weren’t able to get this concentration right until the end of our research sadly.
Are there any limitations to using Betulin as an antibiotic?
Since betulin did come in powder form, I would think a limitation to using it as an antibiotic would be transforming it into the perfect concentration and stock solution that best stops the growth of Salmonella Typhimurium. There needs to be a lot of more testing done, especially in mice and eventually humans to see how this compound and the subject reacts!