Thank you! Removing the color from EGCg will allow the spectrophotometer to more accurately measure the absorbance. The EGCg we used was very dark, which would have caused the spectrophotometer to measure a much higher absorbance, so we had to color match our positive and negative controls but this was not very accurate.
Great job! Can you further elaborate on how the colored powder form of EGCg may have confounded your results? And what results you would expect if you used the liquid, clear form instead?
Thank you! The colored form of EGCg that we used was dark; this would have caused the spectrophotometer to measure a higher absorbance, as less light is able to pass through the solutions. In order to make our results more accurate, we had to color match our positive and negative controls to the dose with the highest concentration. If we used a liquid and clear form of EGCg, we would have much more accurate absorbances and the compound would clearly be acting as an antibiotic. The liquid form would not need to be dissolved in another liquid, like the powder form is.
EGCg in the powder form needs to be dissolved in a liquid before we put it into a plate with bacteria. As we dissolve the powder in a liquid, the solution becomes diluted. The liquid form would not need to be dissolved in anything and would not be diluted, like the powder form is, so the liquid form would have a higher concentration to begin testing with.
Great job! Why would you have the chemistry department remove the color from the EGCg?
Thank you! Removing the color from EGCg will allow the spectrophotometer to more accurately measure the absorbance. The EGCg we used was very dark, which would have caused the spectrophotometer to measure a much higher absorbance, so we had to color match our positive and negative controls but this was not very accurate.
Great job! Can you further elaborate on how the colored powder form of EGCg may have confounded your results? And what results you would expect if you used the liquid, clear form instead?
Thank you! The colored form of EGCg that we used was dark; this would have caused the spectrophotometer to measure a higher absorbance, as less light is able to pass through the solutions. In order to make our results more accurate, we had to color match our positive and negative controls to the dose with the highest concentration. If we used a liquid and clear form of EGCg, we would have much more accurate absorbances and the compound would clearly be acting as an antibiotic. The liquid form would not need to be dissolved in another liquid, like the powder form is.
Why would having the EGCg in a liquid form be better than the dissolvable powder form?
EGCg in the powder form needs to be dissolved in a liquid before we put it into a plate with bacteria. As we dissolve the powder in a liquid, the solution becomes diluted. The liquid form would not need to be dissolved in anything and would not be diluted, like the powder form is, so the liquid form would have a higher concentration to begin testing with.