Would it be worth testing the treatment with other types of bacteria since it showed promise in the 20 mg/mL dosage zone? Or would the effectiveness of the treatment be benchmark for other types of bacteria?
Great Question. It Definitely could be worth looking at other types of bacteria. We would need to complete additional research at the 20mg/mL dose and other doses in S.Typhimurium to establish the best dose, and solidify that EGCg shows antibiotic properties consistently before moving into additional testing. That additional testing could include using other Salmonella bacteria, such as S.Typhi, Then move on to other bacteria, such as streptococcus, as the model organism to determine the effectiveness across multiple strains of bacteria.
Great Question. Additional Dose-response Experiments could be performed along with testing just the 20mg/mL dose following the Max-Dose protocol to compare it just to the Negative and Positive controls and not additional doses. Additionally, a Bacteriostatic/ Bactericidal experiment and a Mueller-Hinton Agar Plate could be completed.
You mentioned that EGCg is easily accessible and inexpensive and can be sold as green tea extract liquid. How does this liquid form of green tea extract differ from the lab-grade EGCg that comes as a liquid?
Great Question. The EGCg sold in most supermarkets is sold as a supplement so it contains many additional vitamins such as Ascorbic Acid (Vitamin C) and Potassium, preservatives such as citric acid, and still contain some of the byproducts of manufacturing since they are not as focused on the purity of the compound. Since lab-grade EGCg is to be used in a scientific and research setting it contains the fewest manufacturing byproducts and does not contain the additives that store bought does so it provides the highest percentage of pure EGCg.
Would it be worth testing the treatment with other types of bacteria since it showed promise in the 20 mg/mL dosage zone? Or would the effectiveness of the treatment be benchmark for other types of bacteria?
Great Question. It Definitely could be worth looking at other types of bacteria. We would need to complete additional research at the 20mg/mL dose and other doses in S.Typhimurium to establish the best dose, and solidify that EGCg shows antibiotic properties consistently before moving into additional testing. That additional testing could include using other Salmonella bacteria, such as S.Typhi, Then move on to other bacteria, such as streptococcus, as the model organism to determine the effectiveness across multiple strains of bacteria.
What tests could someone perform to better determine if the 20 mg/mL EGCg dose could be an antibiotic dose?
Great Question. Additional Dose-response Experiments could be performed along with testing just the 20mg/mL dose following the Max-Dose protocol to compare it just to the Negative and Positive controls and not additional doses. Additionally, a Bacteriostatic/ Bactericidal experiment and a Mueller-Hinton Agar Plate could be completed.
You mentioned that EGCg is easily accessible and inexpensive and can be sold as green tea extract liquid. How does this liquid form of green tea extract differ from the lab-grade EGCg that comes as a liquid?
Great Question. The EGCg sold in most supermarkets is sold as a supplement so it contains many additional vitamins such as Ascorbic Acid (Vitamin C) and Potassium, preservatives such as citric acid, and still contain some of the byproducts of manufacturing since they are not as focused on the purity of the compound. Since lab-grade EGCg is to be used in a scientific and research setting it contains the fewest manufacturing byproducts and does not contain the additives that store bought does so it provides the highest percentage of pure EGCg.