4 thoughts on “D76 -Singh

  1. In the limitations you mention how the absorbance value had a significance difference and how longer centrifugation could help with this. In what specific ways would this help solve the problem?

    1. By increasing particle suspension in the solvent, we would have a more distributed sample of silver nanoparticles in 50% DMSO. What we noticed was that if we had vortexed the sample for approximately 5 seconds, this was not a sufficient amount of time to allow for the particles to mix with the DMSO. Because of this issue, the first few trials to determine the maximum dose were incomplete representations of the silver nanoparticle max dose. Hence, when we increased the time to mix the particles and DMSO to about 15 seconds, we noticed more consistent results and the consistency indicated the bactericidal property of silver nanoparticles.

  2. How would you plan to investigate the toxic effect of silver nanoparticles?

    1. To investigate the potential toxicity of silver nanoparticles, a series of dilutions between 10 and 5 mg/mL could be prepared. The purpose of doing so is to create various doses between 10 and 5 mg/mL, which can be tested in a sample of S. Typhimurium (following the same methodology presented in this presentation) to see what the A620 readings or absorbance values are. This can provide a model in terms of how the silver nanoparticles behave with the bacteria. If a dose is toxic, then we can develop a therapeutic index to compare the therapeutic effect to the toxic effect and determine whether the target compound is safe or not.

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