12 thoughts on “D94 – Fraser

  1. Great Presentation! You were really clear, easy to understand, and made your poster very accessible. Do you think any changes in your protocol would have yielded more significant results? Would you have done anything differently if you could try again?

    1. Hey David, so initially my lab partner and I wanted to test multiple compounds. We started off with a few different elderberry extracts but ultimately only had enough time to test the lab grade one. Yes, I think that we could have yielded more significant results if we had more time to test all of them and if we were able to evenly distribute the larvae between the vials.

  2. Hi Colin, amazing presentation, very well done! I wanted to ask, i believe you stated you believed the compound would have a positive effect on the survival rate of the larvae even in the absence of radiation, (i apologize if ive misheard). What lead you to this hypothesis?

    1. Hey Charlotte, I apologize if I misspoke. So both the positive control (colchicine) and our experiemtal compound (porcyanidin B1) were expected to decrease the survival rates of the flies. and our negative control (DMSO) was expected to not have an affect on the survival rates.

  3. Your presentation was great! It was very clear and I fully understood. Really well done. Why did is that the ideal life cycle to begin?

    1. Hey Morgane, so the 3rd instar larvae portion of the flies life cycle is ideal for cancer research because they exhibit similar characteristics to cancer cells (which in turn are also similar to stem cells, which the flies have in their development). Overall, the fruit flies are a great model organism due to their short life cycle, they are inexpensive, easy to manipulate, produce a ton of offspring, and can be genetically modified with relative ease.

  4. I agree with everyone else that this was a good presentation. I noticed you kept referring to the word “hit,” but I was not too sure what it means- I am sorry if you stated it in the video.

    1. Hey Sam, no worries, so to develop our line in our graph we used class data that we gathered with a couple hundred data points to create the definition of a hit. The line represents 2 standard deviations below the mean survival rate (which was approximately 15%). Any vial that we had in our experiment that had a survival rate below the 2 standard deviations would be considered a ‘hit’, meaning it could be a potential chemotherapeutic.

    1. Hey Naomi, so fruit flies are an ideal substitute for actually studying cancer cells because they are relatively inexpensive, they are easy to culture in labs, they reproduce a ton of offspring, have a short lifecycle, low number of chromosomes and can be genetically modified with comparative ease. Specifically, we used the 3rd instar larvae portion of their life cycle.

  5. What’s a compound that you could test similar to the one already tested that would maybe provide a different result? Since there was not a hit, it might be better to test something different.

    1. Hey Nolan, so a similar compound that we could test in the future would be other types of proanthocyanidins (from catechin and epicatechin molecules). The reason we chose to study this specific compound is because it has been researched in the past and has shown to have significant effects on inter-cellular communication. I agree perhaps next time we could spend some more time researching potential candidates for chemotherapy.

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