8 thoughts on “D99 – Holliday

  1. If the max possible dose absorbable by a person has a great effect on salmonella growth and your data shows that it is in fact bacterialstatic, why would you want to find the minimum effective dose?

    1. Hi Pam, great question! The best answer I would have for this is that even though the compound is bacteriostatic, it can still effect human cells in a negative manor. This means that the max doses are highly probable to be toxic in human cells, which could be learned about more in future studies and experiments. Therefore, we would want to use the lowest dose that still has a positive effect on inhibiting the growth of the bacteria to limit the side effects that the drug may cause on human cells.

    1. Thank you for the question Alli. So a bacteria can be identified by either being gram positive or gram negative. Gram negative bacteria have a thin peptidoglycan layer in their cell wall whereas gram positive bacteria have a thick layer of peptidoglycan layer in their cell wall. This causes gram positive bacteria to have a thinner outer cell wall when compared to gram negative bacteria. The thicker gram negative cell wall makes that bacteria more resistant to antibiotics because of the difficulty to penetrate the cell wall meaning the antibiotics have to find other mechanism to kill the bacteria.

  2. Why are treatments that have an absorbance below 2 std considered effective at preventing bacterial growth?

    1. Hi Cornelius! Absorbances below 2 standard deviations are considered a statistical hit. This standard deviation is based off the mean absorbance of the bacteria growth in the negative control, so anything outside of this range shows that it has potential antimicrobial properties. Therefore anything outside 2 standard deviations represents a hit as it is showing it can effectively kill/inhibit the growth of S. Typhimurium.

    1. Hi Erica! Thank you for the question. The absorbances are showing salmonella growth in each well plate. It is labeled as absorbance because the salmonella is growing and absorbing into the media in the well and that is how each plate is read. Therefore, the absorbance patterns showing which doses allow or don’t allow the bacteria to grow are related to the experimental results as they show which dose concentrations are affective against inhibiting the growth of the bacteria. These concentrations can then be tested further.

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