We ran more dose response curve tests to try and get more data on the various concentrations absorbance values to hopefully help get more data that backed up one of the two contradicting plates so we could then figure out which one could be considered “bad data” and which one we could use as “good data”.
A hit is determined by first taking the mean of the negative control, in this case DI water, absorbance values and then figuring out the standard deviation of the negative controls absorbance values. You then do the mean plus 2 times the standard deviation for your upper value, and the mean minus 2 times the standard deviation for your lower value. Absorbance values above this upper value are potential hits for probiotics as they promote bacterial growth compared to the negative control. Absorbance values below the lower value are potential hits for antibiotics as they kill more bacteria compared to the negative control. These values are significant as they fall outside of the 95 percent inside of these 2 standard deviations when looking at a normal distribution curve, therefore making them statistically significant.
Good presentation. For your future directions, would you plan on using different mediums for the different bacterias that you would use. Also, would you use a different negative control such as ethanol to see if your results change dramatically with a different negative control.
The type of negative control is dependent of the compound you are using. The purpose of the negative control is to figure out what would happen if nothing changed during the experiment which in this case would just be normal salmonella growth in the certain negative control. The compound must also be soluble in the negative control as this is the solution we are dissolving the compound in and therefore must figure out what happens to the salmonella with the absence of the compound while still using the material used to dissolve the compound. Therefore different compounds would have different negative controls and for naloxone we would only be using DI water, no matter the bacteria it is being used to potentially act as an antibiotic on. So ultimately yes, the data would change drastically if we used something such as ethanol as the negative control, but for the integrity of the research it would never be used as the negative control.
Good presentation. For your future directions, would you plan on using a different negative control such as ethanol to determine if your results change dramatically?
No because the negative control is picked based upon compound you use so therefore we wouldn’t use any other negative control besides DI water unless we decided to use a different compound in which we would change the negative control.
The spectrophotometer that we used on all of our plates determines the amount of light absorbed by the bacteria in the different wells. Therefore if you have an antibiotic and more bacteria die then you will get a lower absorbance value as the bacteria are absorbing less light due to being killed off. Conversely, if you have a probiotic and get more bacterial growth then you will get a higher absorbance value as the bacteria are absorbing more light as there is more bacteria now in the well absorbing the light.
The spectrophotometer that we used to measure the absorbance values looks at how much light is being absorbed by the bacteria in each well of the plate. Therefore if there is more bacterial growth in the wells, then more light will be absorbed by the bacteria and you will therefore get a higher absorbance value.
curesymposium.org 
What tests did you run when you noticed some of the plates had absorbance values that were contradictory to each other?
LikeLike
We ran more dose response curve tests to try and get more data on the various concentrations absorbance values to hopefully help get more data that backed up one of the two contradicting plates so we could then figure out which one could be considered “bad data” and which one we could use as “good data”.
LikeLike
What is a Hit and why is it relevant?
LikeLike
A hit is determined by first taking the mean of the negative control, in this case DI water, absorbance values and then figuring out the standard deviation of the negative controls absorbance values. You then do the mean plus 2 times the standard deviation for your upper value, and the mean minus 2 times the standard deviation for your lower value. Absorbance values above this upper value are potential hits for probiotics as they promote bacterial growth compared to the negative control. Absorbance values below the lower value are potential hits for antibiotics as they kill more bacteria compared to the negative control. These values are significant as they fall outside of the 95 percent inside of these 2 standard deviations when looking at a normal distribution curve, therefore making them statistically significant.
LikeLike
Good presentation. For your future directions, would you plan on using different mediums for the different bacterias that you would use. Also, would you use a different negative control such as ethanol to see if your results change dramatically with a different negative control.
LikeLike
The type of negative control is dependent of the compound you are using. The purpose of the negative control is to figure out what would happen if nothing changed during the experiment which in this case would just be normal salmonella growth in the certain negative control. The compound must also be soluble in the negative control as this is the solution we are dissolving the compound in and therefore must figure out what happens to the salmonella with the absence of the compound while still using the material used to dissolve the compound. Therefore different compounds would have different negative controls and for naloxone we would only be using DI water, no matter the bacteria it is being used to potentially act as an antibiotic on. So ultimately yes, the data would change drastically if we used something such as ethanol as the negative control, but for the integrity of the research it would never be used as the negative control.
LikeLike
Good presentation. For your future directions, would you plan on using a different negative control such as ethanol to determine if your results change dramatically?
LikeLike
No because the negative control is picked based upon compound you use so therefore we wouldn’t use any other negative control besides DI water unless we decided to use a different compound in which we would change the negative control.
LikeLike
Why does higher absorbance of the sample correlate with the bacterial growth?
LikeLike
The spectrophotometer that we used on all of our plates determines the amount of light absorbed by the bacteria in the different wells. Therefore if you have an antibiotic and more bacteria die then you will get a lower absorbance value as the bacteria are absorbing less light due to being killed off. Conversely, if you have a probiotic and get more bacterial growth then you will get a higher absorbance value as the bacteria are absorbing more light as there is more bacteria now in the well absorbing the light.
LikeLike
The spectrophotometer that we used to measure the absorbance values looks at how much light is being absorbed by the bacteria in each well of the plate. Therefore if there is more bacterial growth in the wells, then more light will be absorbed by the bacteria and you will therefore get a higher absorbance value.
LikeLike
Sorry for this extra comment, on my screen it had shown up that the first comment didn’t go through so I also wrote this one.
LikeLike