14 thoughts on “G1 – Beato

  1. Hi! I enjoyed your poster and presentation! My question is if this research showed promising results with future experiments, what would this effect in the overall picture of the research? What is the overall purpose of this research? Thanks, and Good job !

    1. While the benefits are largely speculative, being able to understand and control the transition from lysogeny to lysis could potentially result in valuable antiviral medications. Blocking the ATPase activity or inhibiting the ClpX protease could keep prophage induction from being initiated.

  2. Hello! Can you explain what “coding potential” is (in the discussion section, you mention that gene 67 has “a greater coding potential”)? Thank you!

    1. Yes! So coding potential is measured by by a bioinformatics program called Starterator. It essentially measures the likelihood that a particular open reading frame would be transcribed.

  3. What diseases do mycobacteria cause and how would the phage be administered to a patient?

    1. Mycobacterium are responsible for for many diseases including Haran’s Disease (leprosy) and tuberculosis. There are many ways by which phages are administered to patients including orally, through intramuscular injection, or topically.

  4. Would there have been any difference in results that would have resulted in you rejecting the null hypothesis?

    1. Excellent question! Short of a BLAST result with significant E-values of a similar ATPase active Clp subunit, not really. Higher e-values of the ATP active domains would have provided more evidence, but likely would not have rejected the null hypothesis. This project was more laying the groundwork for future experimental research to uncover the identity of the ATP active Clp subunit.

    1. The catalytic triad is the triad of Aspartate, Histidine, and Serine residues within the enzyme that are responsible for proteolytic activity.

  5. Hey Quinn, great presentation. What is a shine dalgarno score and why does your score support your counter argument. What is a range of good values? Bad values?

    1. A Shine-Dalgarno sequence is a genomic sequence that the ribosome binds to in order to initiate translation. The higher the Shine Dalgarno score, the more likely it is that the gene is transcribed. An excellent SD score would be -1, while a poor SD score might be -10.

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