You mentioned expanding your search outside of the m1 cluster for other cases of lateral gene transfer –what else would you look at if you were to continue the study? Awesome presentation, by the way!
Upon finding a phage that infects both bacteria I would recreate the pathway by using plasmid DNA from one bacteria and inserting it into the phage. Then I would infect the other bacteria with the phage with this plasmid and hope to see some sort of recombination in the bacteria with the phage, specifically with this plasmid. This phenomenon does happen (although quite rarely) within temperate phage.
if I were to find a phage that infects both M. smeg and E. coli, I would insert plasmid DNA from one of the bacteria into the phage using plasmids. From there I would infect the other bacteria with the phage (and plasmid) and see if there was recombination. While this is a relatively rare phenomenon, it does occur with temperate phages.
Thanks! If I were expanding my research to outside of the M1 cluster, I would look for clusters with phages know to infect more than one type of bacteria. This would help me re-create the pathways of LGT.
Some experiments I could do to further the research on this topic would be the characterization of how the phage takes the DNA from the bacteria. I assume this is due to a malfunction in the machinery when the phage is replicating and packaging the DNA for the new phage.
Only one direction! The phage is able to transfer the DNA from one bacteria to another, it would be very odd to have a phage transmit DNA from a single type of bacteria back to itself.
You mentioned expanding your search outside of the m1 cluster for other cases of lateral gene transfer –what else would you look at if you were to continue the study? Awesome presentation, by the way!
If you were to find a phage that infects both, how would you replicate the pathway and what would that process look like?
Upon finding a phage that infects both bacteria I would recreate the pathway by using plasmid DNA from one bacteria and inserting it into the phage. Then I would infect the other bacteria with the phage with this plasmid and hope to see some sort of recombination in the bacteria with the phage, specifically with this plasmid. This phenomenon does happen (although quite rarely) within temperate phage.
if I were to find a phage that infects both M. smeg and E. coli, I would insert plasmid DNA from one of the bacteria into the phage using plasmids. From there I would infect the other bacteria with the phage (and plasmid) and see if there was recombination. While this is a relatively rare phenomenon, it does occur with temperate phages.
Thanks! If I were expanding my research to outside of the M1 cluster, I would look for clusters with phages know to infect more than one type of bacteria. This would help me re-create the pathways of LGT.
What experiments would you use to further conduct research on this topic?
What experiments would you use to further your research on this topic? What information is necessary?
Some experiments I could do to further the research on this topic would be the characterization of how the phage takes the DNA from the bacteria. I assume this is due to a malfunction in the machinery when the phage is replicating and packaging the DNA for the new phage.
Can lateral gene transfer work in both directions or only one?
Only one direction! The phage is able to transfer the DNA from one bacteria to another, it would be very odd to have a phage transmit DNA from a single type of bacteria back to itself.