9 thoughts on “G21 – Topping

  1. You mentioned that you theorize that it might be beneficial for the gene to be inverted in phage, what potential benefit could this provide?

    1. You can see in my reply to Gabrielle why we theorize the inversion is beneficial or at least not harmful to the phage! As far as the ways in which it could change the function of the tail protein, there are a lot of possibilities but I think the most likely is that the inversion would allow for faster assembly of the tail in progeny phage as the inversion is technically in a chaperone protein.

  2. Are the inversions seen in the Q cluster evident in other clusters as well? Why would the inversion be beneficial in the phage, meaning what is the thought process behind that statement?

    1. Hi Gabrielle!
      We actually don’t know if the inversion is beneficial but since this phage was isolated from a random soil sample we can assume it is at least not harmful enough to decrease pathogenicity significantly. More research is needed to find out exactly how the inversion affects the phenotype of the phage!

    2. And these inversions specifically are not seen in other sub clusters although other clusters do sometimes have inversions in the tail protein genes.

  3. Hey Emma,

    You mentioned a few times that the tail gene was inverted. I might’ve missed it but can you clarify what inverted means? Is it just running backwards from the expected reading frame of the rest of the genes?

    Thanks,
    Jake

    1. Yes that’s correct! It should also be noted that this gene can only be read in the “backwards” frame since it does not seem to produce any protein product when translated normally.

    1. A cluster is a group of phage with 75% genome similarity and the significance of this is that looking at genetically similar organisms with small differences makes it easier to pinpoint what those differences do!

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