It is hard to say if it would have affected it, it could cause the gene to be non-functional, which is the reason for studying this gene in the role of lysogeny from the beginning.
You mention that the amino acid changes did not affect the function of integrase. If this conclusion were reversed, and there was an impact on integrase, how would this change your future research direction?
There were changes, not many, but the changes that occurred were not changes that could likely cause a change in the function of the gene. Meaning, it could have changed from a positive amino acid to another positive one or polar to polar. This would not likely change how the protein folds, thus affecting the role of integrase.
You mention that the amino acid changes did not affect the function of integrase. If the situation were reversed and the changes were effective in altering the function of integrase, how would this finding affect your future research?
This would be more of what I was looking to see when I was doing my research. There is a gene encoding for a protein in the genome of Juniormint that causes it to enter the lysogenic cycle and be temperate. If the function of integrase was inhibited by amino acid changes in the functional region, this could be a possible reason for the phage being temperate but having lytic morphology.
You mention that the amino acid changes did not affect the function of integrase. If this conclusion were reversed, and there was an impact on integrase, how would this change your future research direction?
If the proline to arginine swap had been made within the functional region, do you know what effect it would have?
It is hard to say if it would have affected it, it could cause the gene to be non-functional, which is the reason for studying this gene in the role of lysogeny from the beginning.
When looking at the dna sequence, was there any change in protein within the region of interest?
You mention that the amino acid changes did not affect the function of integrase. If this conclusion were reversed, and there was an impact on integrase, how would this change your future research direction?
There were changes, not many, but the changes that occurred were not changes that could likely cause a change in the function of the gene. Meaning, it could have changed from a positive amino acid to another positive one or polar to polar. This would not likely change how the protein folds, thus affecting the role of integrase.
You mention that the amino acid changes did not affect the function of integrase. If the situation were reversed and the changes were effective in altering the function of integrase, how would this finding affect your future research?
This would be more of what I was looking to see when I was doing my research. There is a gene encoding for a protein in the genome of Juniormint that causes it to enter the lysogenic cycle and be temperate. If the function of integrase was inhibited by amino acid changes in the functional region, this could be a possible reason for the phage being temperate but having lytic morphology.
You mention that the amino acid changes did not affect the function of integrase. If this conclusion were reversed, and there was an impact on integrase, how would this change your future research direction?