What a beautiful poster! Do you think that changing the shine-delgarno sequence in future research would affect the efficiency of the adjacent start codon at all? Possibly affecting the ability to translate?
Hi Hannah! Thank you so much 🙂 I think that this could definitely be a possibility! One of the factors that we looked at for the start codon was the score of the SD Sequence, so it could be a possibility that lowering the score would impact the start codon and the ability for it to translate. Great question!
LOVE your poster, it is amazing! I was wondering what kind of results would be expected if the slippery sequences were mutated or not present in the phage genome?
Thank you! 🙂 If the slippery sequences were mutated, there is a possibility that ribosomal frameshifting would not occur. This is because ribosomal frameshifting is heavily dependent on the slippery sequence. Every phage has a specific slippery sequence that is indicative of frameshifting. Therefore, if these weren’t present or mutated in the phage genome, ribosomal frameshifting might not occur. If ribosomal frameshifting doesn’t occur, it might impact the downstream sequences and coding of other tail proteins. The ribosomal frameshifting specifically occurred for tail assembly chaperone proteins, so without it a successful tail might not be built.
If there was a mutated sequence, it would be likely that ribosomal frameshifting might not occur. This is because ribosomal frameshifting is heavily dependent on the slippery sequence. Further research has been done in other viruses that propose that a SD sequence upstream of the slippery sequence can also impact its ribosomal frameshifting mechanism. Further research needs to be done on the impact that the SD sequence can have on phage ribosomal frameshifting. It could also be a possibility that altering the SD sequence can change the type of ribosomal frameshifting that occurs. There are essentially three different types of ribosomal frameshifting. Understanding and researching this component can be really helpful in better understanding the mechanisms of ribosomal frameshifting and the factors that determine it’s type.
What a beautiful poster! Do you think that changing the shine-delgarno sequence in future research would affect the efficiency of the adjacent start codon at all? Possibly affecting the ability to translate?
Hi Hannah! Thank you so much 🙂 I think that this could definitely be a possibility! One of the factors that we looked at for the start codon was the score of the SD Sequence, so it could be a possibility that lowering the score would impact the start codon and the ability for it to translate. Great question!
LOVE your poster, it is amazing! I was wondering what kind of results would be expected if the slippery sequences were mutated or not present in the phage genome?
Thank you! 🙂 If the slippery sequences were mutated, there is a possibility that ribosomal frameshifting would not occur. This is because ribosomal frameshifting is heavily dependent on the slippery sequence. Every phage has a specific slippery sequence that is indicative of frameshifting. Therefore, if these weren’t present or mutated in the phage genome, ribosomal frameshifting might not occur. If ribosomal frameshifting doesn’t occur, it might impact the downstream sequences and coding of other tail proteins. The ribosomal frameshifting specifically occurred for tail assembly chaperone proteins, so without it a successful tail might not be built.
What would happen if there were mutated sequences? What would be the most common/relevant sequences to occur?
If there was a mutated sequence, it would be likely that ribosomal frameshifting might not occur. This is because ribosomal frameshifting is heavily dependent on the slippery sequence. Further research has been done in other viruses that propose that a SD sequence upstream of the slippery sequence can also impact its ribosomal frameshifting mechanism. Further research needs to be done on the impact that the SD sequence can have on phage ribosomal frameshifting. It could also be a possibility that altering the SD sequence can change the type of ribosomal frameshifting that occurs. There are essentially three different types of ribosomal frameshifting. Understanding and researching this component can be really helpful in better understanding the mechanisms of ribosomal frameshifting and the factors that determine it’s type.