11 thoughts on “P32 – Munch

  1. What does it mean when you say your phage was isolated through enrichment? (I think that is the word you used, but I couldn’t hear exactly.)

    1. yes! By enrichment I mean we took a soil sample (which could have tons of different phage in it) and then did dilutions on that sample until we hopefully only had one phage left.

    1. phage therapy can be used on many types of diseases where cells are acting abormally. It is better than alot of treatments we have now like chemotherapy because chemo kills all cells present whereas phage will only kill cells that are the issue which ends up being much less harmful to the body. In order to choose a phage we use a bacteria that is similar to the disease and see if the phage can infect it. M.Smeg is very similar to TB, because it is dangerous to have TB laying around in a lab we use M.Smeg to see if our phage can infect it. If the phage is able to infect the bacteria that is the phage we will choose. If it is not able to infect the bacteria then it will not be able to infect the disease and will not be helpful.

  2. Nice job on the presentation! When you said that your phage was contaminated with Corndog is that another phage that was isolated in this class or is it a previously characterized known phage?

    1. Corndog is a previously characterized phage. Unfortunately alot of people in the class had the same type of contamination so we are beginning to believe that corndog (the contaminant) could be airborne.

    1. because there is a huge phage database, we could have used what we saw on the restriction digest and match it to one that is in the database to see what cluster that phage is in. a cluster allows us to characterize a phage a little more than just what it looks like and it can help us narrow down the uses for that phage which can be helpful for future research.

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