12 thoughts on “P1 – Mutter

  1. Thank you for your presentation. What about phage therapy allows it to be used instead of antibiotics?

    1. Antibiotics role is to kill bacteria cells, phage can essentially perform the same function by invading bacteria and killing the bacterium from within.

  2. What does poppy’s “O cluster” shape tell you about the phages functions with infecting and what kind of therapy it could be potentially used for?

    1. Awesome presentation! In regards to your future directions and future testing, do you expect to see a significant difference in DNA degradation in comparison to your experiment?

      1. Yes with further testing, we would redo DNA isolation in order to get more concentrated and quality DNA to use for PCR which will help us get clearer and more reliable results.

    2. Poppy’s O cluster shape tells us that this phage has a long tail which is useful for inserting viral DNA into the bacteria cell. Poppy can be used for phage therapy which is a replacement for antibiotics in bacteria infections health problems wise.

    3. Poppy’s O cluster shape tells us that this phage has a long tail which is useful for inserting viral DNA into the bacteria cell. Poppy can be used for phage therapy which is a replacement for antibiotics in bacteria infections health problems wise.

  3. Was Poppy a clear O cluster (can they be more than one cluster/sub cluster) or could it be similar to another cluster thus having multiple functions?

    1. Yes Poppy is an O cluster phage and there can be a sub cluster but we were not able to identify its specific sub cluster. When a phage is in a cluster, it means that they have similar functions and genes to one another.

    2. Poppy was an O cluster phage and we were not able to identify the specific sub cluster, but phages in the same cluster and sub cluster will have similar functions and genes.

  4. Great presentation and really cool to see application of PCR. I believe you mentioned that the PCR was unsuccessful, what would you look for to determine if a future PCR was successful?

    1. Future PCR that is successful would show more clear straight short bands rather than a long streak on the agarose gel.

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