It was based mostly on the tail length, head size, and where the tail fibers were placed, the most common types of phages all look very different because of their tail length and fibers so its pretty easy to classify a phage based on what it looks like
The other 3 enzymes did not cut the DNA. In other phages it is possible that they will, and comparing which enzymes cut the DNA and how many times the DNA was cut is what allows this restriction digest to help form a hypothesis on what cluster and subcluster a phage might be apart of
I’m not entirely sure of the a specific name for it, but there were some groups in this lab who were able to take primers that they new amplified the DNA of certain clusters of phages, which is what I was imaging could be used on this phage
Hi Katie, from reading the caption of the EM photo, what are the parameters that you needed to know that ConkreeT was a siphoviridae phage?
It was based mostly on the tail length, head size, and where the tail fibers were placed, the most common types of phages all look very different because of their tail length and fibers so its pretty easy to classify a phage based on what it looks like
I thought phages were only able to infect one type of bacteria? How would it work that you do work with M. tuberculosis instead of M. Smeg?
Depending on the phage, some are able to infect many different bacteria especially if the bacteria are closely related
You mentioned you used 6 enzymes, 3 of which cut the DNA. What do the other three do?
The other 3 enzymes did not cut the DNA. In other phages it is possible that they will, and comparing which enzymes cut the DNA and how many times the DNA was cut is what allows this restriction digest to help form a hypothesis on what cluster and subcluster a phage might be apart of
What type of PCR would be the best to use for this?
I’m not entirely sure of the a specific name for it, but there were some groups in this lab who were able to take primers that they new amplified the DNA of certain clusters of phages, which is what I was imaging could be used on this phage