8 thoughts on “P60 – Nguyen

  1. Do you think certain soil types or different climates impact the viability/types of bacteriophages?

    1. Hi Evie! Different species of bacteria have different ideal living conditions, and so soil type and climate would affect the populations of bacteria living in the area. Since bacteriophage are only able to replicate within certain bacteria, you can expect that the most common phage in a specific area will be the ones able to infect the bacteria most capable of living there. As for viability, I can’t say for sure, but I assume it’s possible that environmental stressors will influence the amount of methylation on certain genes in the virus, which will affect which traits are expressed.

    1. Hi Kenya! Our main limitation was time; this lab was very time-consuming and had a lot of moving parts, so any set-backs at the beginning meant we wouldn’t be able to get to any of the later experiments. My partner and I ran out of time to perform a restriction digest and PCR experiment on our isolated phage, and as a result were unable to determine its cluster.

  2. Do you think that there could be any uses for this phage as a temperate phage instead of changing it into a lytic one?

    1. Hi Addison! Temperate phage are very useful in instances where the bacteria needs to be preserved (i.e. not lysed and killed). An example would be modifying a phage so that it expresses a protein that glows green when it is integrated into a host; this phage could be used to identify whether or not a certain bacteria is present in a sample. If the bacteria is present, the phage will infect and integrate its DNA into the bacterial genome, expressing the protein and glowing green under observation.

  3. Hello!

    I had a question on how you would modify the phage into order for it to kill bacteria immediately. What part of the phage would have to be changed? Would it even be possible for a phage that is temperate to be changed into a phage that acts more lytic?

    1. Hi Stephanie! Yes, it is possible for a temperate phage to be genetically modified into a lytic phage. Temperate phage can go through both the lysogenic and lytic cycle, and so they have all the genes of a lytic phage–they just also have the extra lysogenic genes as well. Temperate phage have a repressor gene that codes for repressor proteins that bind to the lytic promoter, preventing any lytic genes from being transcribed; this is how they are able to integrate into the bacterial genome and avoid lysing/killing the host cell. When temperate phage exit the lysogenic cell and go through the lytic cell, they simple stop expressing the repressor gene. To ensure the phage will go straight to the lytic cycle, all you would need to do is remove the repressor gene from the viral genome (and maybe the integrase gene as well, to be safe).

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