8 thoughts on “P61 – Campbell

  1. Is there any evidence to suggest which phage was lytic and which was temperate, or is it entirely unclear?

    1. we have no evidence to suggest which phage is lytic and which is temperate. We would need to isolate the three phages and sequence their DNA in order to determine which is which.

  2. How would you change how you have been isolating your phages in order to isolate only a single one? Also you claim to have isolated three different phages but you only picture two, why is that?

    1. We would continue serial dilutions with lower concentrations of the High Titer Lysate, after plating and incubation we would pick the smallest plaques until the plaque morphology is consistent. In regards to the missing picture of the 3rd phage, we couldn’t fit it on the poster.

  3. I took this class before, our lysate was surprisingly pitch black. I wonder what color of your HTL? In addition, I think it is a great idea to separate three phages and study them individually; thereby, we can learn how each phage interact and affect the bacteria.

    1. wow, our HTL was clear… with maybe a slight hint of yellow, which was consistent with the rest of my lab section. The department possibly changed a component of the materials which could explain the variation between them.

    1. We expected the 3 phage to be in different clusters and different lifecycles. However, we could only concluded they’re within the same family (siphoviridae) and have different lifecycles (we were unable to determine which lifecycle corresponded with the different phage.)

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