You said the O-Clusters are relatively rare, and our knowledge is limited, what do you think learning more about O Cluster Phages would contribute to medical science? (What would this knowledge be able to be applied to in humans, (medicines, therapies etc.)
It would be helpful to know whether O-cluster phage are generally lytic or temperate as well as their host-range in order to use them in phage therapy.
How does purification work (i.e., How does it maintain the aspects of phage that you want to study? What are the technical steps of purification?) Also, why are two rounds of purification necessary?
When plating a lysate to form plaques, each circular plaque is the result of a single active phage or plaque forming unit. By using a single plaque to form the lysate for the next plate we can ensure some purity of that next lysate. We have two rounds of purification because it’s possible that there was some overlap of plaques on our first plate, so we used the second plate to ensure purity.
Were there other clusters that your temperate phage matched in the similarity of homology, since you described O-cluster as rare?
Nope. The only cluster with this distinctive morphology is O-cluster.
You said the O-Clusters are relatively rare, and our knowledge is limited, what do you think learning more about O Cluster Phages would contribute to medical science? (What would this knowledge be able to be applied to in humans, (medicines, therapies etc.)
It would be helpful to know whether O-cluster phage are generally lytic or temperate as well as their host-range in order to use them in phage therapy.
Is there a specific sub cluster that is more common for O-cluster phage? Do you have an expected sub cluster for the phage?
O-cluster does not currently have any known subclusters as there are only 21 documented O-cluster phages
How does purification work (i.e., How does it maintain the aspects of phage that you want to study? What are the technical steps of purification?) Also, why are two rounds of purification necessary?
When plating a lysate to form plaques, each circular plaque is the result of a single active phage or plaque forming unit. By using a single plaque to form the lysate for the next plate we can ensure some purity of that next lysate. We have two rounds of purification because it’s possible that there was some overlap of plaques on our first plate, so we used the second plate to ensure purity.