8 thoughts on “P63 – Hogue

  1. How does sequencing of DNA for tail fibers allow you to determine host range of a phage?

      1. Some clusters are just formed more rarely. Cluster A, for example, is incredibly common; there are several sub clusters and tens of individual species under each sub cluster. Other clusters just have a more unique morphology that occurs less frequently. If you’d noticed, the majority of P section ended up with O cluster; but this is an even more rare phenomenon caused by contamination by this unique phage.

    1. Phage attach to external sites on bacteria by way of their tail fibers; it’s been found in experimentation that there are frequently similarities between phage with commonalities in sequencing around the tail fibers being an indicator of which binding sites they can affect.

  2. Excellent project, something I was unclear about was what the purpose and the findings of the electrophoresis was; could you explain that further?

    1. Absolutely; gel electrophoresis is used to determine the presence of DNA by catching it in the netted structure of the .8% gel. If further experiments had been successful, nucleases would cut DNA at specific points, producing X amount of smaller pieces of DNA. The number of cuts and which nucleases were effective would be an indicator of the cluster of the present phage. However, our DNA was deteriorated and produced no readable data from the nuclease cut.

    1. The dairy industry research used sequenced phage to identify similarities in the genomes of phage that attacked desired bacteria used in milk, cheese, and yogurt production. In doing so, they noticed that it was sequences related to tail fibers that could be used to determine the specific bacteria they attack. Since week two of this section, I’ve been fascinated by the idea of using sequenced phage genomes to find the similarities or patterns of genomic sequences to assign them to which binding sites and therefore which bacteria they could/would attack/affect in an effort to design a database that would allow quick selection of phage useable on any bacteria with known binding sites without the months-nay-years of experimentation currently necessary to determine effective phage.

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