8 thoughts on “P64 – Polatsek

  1. Hello!

    Well done! One question I have for you is do you foresee ways that scientists could alter temperate bacteriophages in order to prevent the risk of increased mutation rates?

    1. Temperate phages use a protein called an integrase that if it were to be removed from the phage’s genome, would prevent the phage DNA from being inserted into the bacterial genome, thus preventing the lysogenic cycle which is what leads to the bacteria being resistant which is that mutation.

  2. For future tests, is there anything you would change doing the DNA isolation, quality control, and restriction digest when trying to figure out which cluster this phage belongs to?

    1. Essentially, the reason our restriction digest didn’t work out as intended is because my partner and I did not have time to perform a quality control gel. This quality control gel is what tells us if the phage is high quality and quantity through a distinct bright band. If there isn’t a bright, distinct band on the quality control gel you would go back and re perform the DNA isolation to hopefully get that high quality and quantity DNA that is needed to get the desired results in the restriction digest that can give you insight into what cluster the phage could be in through similar cuts from the restriction enzymes in your phage and a phage in a cluster.

  3. How are the lytic and lysogenic life cycle different? whats occurs that makes them show different plaques

    1. For the lytic cycle, the bacteria cell immediately starts making the phages proteins and DNA that form the virions that are released when the cell lyses. But the lysogenic cycle has the phage DNA inserted into the genome of the bacteria itself, allowing the phage DNA to replicate with the bacteria and the bacterial DNA. Basically, the lysogenic life cycle has the phage DNA being incorporated into the bacterial genome and in the lytic you do not, it goes directly into copying the phage DNA and making the phage proteins.

    1. The larger the plaque indicates a higher rate of replication of the phage because it is able to kill a greater amount of bacteria in the incubation time allotted. Basically, the faster the phage can infect the bacteria and kill it the faster the new phages made in that bacteria can go on to infect other bacteria and so on in the same amount of time of incubation.

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