7 thoughts on “P65 – Pritchard

  1. Thanks for your work. One comment I have is that it seems like restriction digest is never explained.

    1. Sorry about that! Me and my lab partner were not able to get to that experiment unfortunately, so I apologize for forgetting to describe it… but it is where we take enzymes that are able to cut DNA at specific sequences, cut DNA from our isolated phage and run these fragments on a gel. These gels allow us to make predictions of the cluster of our phage, which are groups of phage that have at least 50% similarity in their genomes. The idea behind the restriction digest is that if two phage genomes are similar enough, the restriction enzymes will cut the DNA into similarly sized fragments. The resulting gels will look similar if the genomes are similar and we can make claims about our phage’s cluster based on the restriction digests that we compare it to (which is from phages with known clusters).

  2. I enjoyed your presentation! The only question I have is what were the limitations present throughout the project?

    1. One of our biggest limitations was time. We had many experiments that we wanted to perform to further our understanding of Grotchard, but we were unable to complete them. We kept running into issues with our purification and making sure that we were really looking at just one phage. As for the resources available for the lab, we were well equip to isolate and analyze our phage.

  3. I really enjoyed your presentation! The one question I have is where there any limitations present throughout the project?

  4. I liked your presentation! What would be other lawns of bacteria that you would test the phage with?

    1. I think it would be best to test Grotchard on bacteria that are extremely antibiotic resistant and are posing an immediate health crisis. One example may be Mycobacterium Tuberculosis. If we could identify Grotchard as being able to infect these bacteria, it may become a critical candidate for potential phage therapy.

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