I think that I would pick from a different plaque since like I mentioned we were having a hard time with our quality control DNA protocol meaning there was no DNA in the sample that we chose, just picking a different plaque all together might have helped with that and also determine if there was any sign of DNA at all.
I think one of the things that went wrong was our filtering process since my lab partner and I had a hard time going through the protocol in the first place and adding the ETA solution, we had to go through 3 rounds of filtering so it could have gone wrong at any time during that. There was also a time where we thought our top agar solution was contaminated.
It doesn’t necessarily effect anything, typically phages range from 24-200 nm we want to make sure its at least big enough to see from the naked eye since and big enough that we can run a pipette tip over to collect it as a sample anything smaller than that we wouldn’t consider a phage for for experiments. if the phage is big enough as well we can see if the phage is lytic or lysogenic as well.
If there is one thing you would change about your experiment, what would it be?
I think that I would pick from a different plaque since like I mentioned we were having a hard time with our quality control DNA protocol meaning there was no DNA in the sample that we chose, just picking a different plaque all together might have helped with that and also determine if there was any sign of DNA at all.
Why does the nucleus need to be deactivated? Were you looking for more plaques?
EDTA solution that we use in the filtering process inactivates nuclease by chelating metal ions the nucleases require.
Do you happen to know what went wrong with your experiment? Is there any limitations you encountered or anything you would do differently?
I think one of the things that went wrong was our filtering process since my lab partner and I had a hard time going through the protocol in the first place and adding the ETA solution, we had to go through 3 rounds of filtering so it could have gone wrong at any time during that. There was also a time where we thought our top agar solution was contaminated.
How does the size of the phage effect the function or shape of the Phage?
It doesn’t necessarily effect anything, typically phages range from 24-200 nm we want to make sure its at least big enough to see from the naked eye since and big enough that we can run a pipette tip over to collect it as a sample anything smaller than that we wouldn’t consider a phage for for experiments. if the phage is big enough as well we can see if the phage is lytic or lysogenic as well.