10 thoughts on “P66 – Kokoshinskly

    1. The difference between a myoviridae and siphoviridae is that a myoviridae has a shorter tail but consists of both long and short tail fibers. A siphoviridae has a longer tail but only consists of one length of tail fibers. They both contain a capsid head with the DNA inside.

  1. Is there any specific research or medicinal purposes that you are hoping your research will be utilized for? In addition, what are the stressors that can cause the lysogenic –> lytic conversion?

    1. I hope our phage would be used in a phage cocktail in order to infect and destroy antibiotic resistant bacteria. Some examples of stressors that can cause the lysogenic cycle to move to the lytic cycle would be starvation and toxic chemicals.

  2. What are the stressors that can induce the lysogenic cycle to move into the lytic cycle? In addition, is there a specific research or medicinal purpose that you hope your research go to?

    1. Two examples of stressors that can induce the lysogenic cycle to move to the lytic cycle would be starvation or toxic chemicals.

      With the research we conducted, I hope that our phage would be used in a phage cocktail to combat antibiotic resistant bacteria in which our phage would be able to infect and destroy the bacteria cells.

  3. In your presentation you point to Figure 4 and mention that “since you can see these bands right above here, this means that we were able to successfully isolate our phage DNA.” What results would indicate that DNA isolation was not successful? Does band length (bp) matter here or do bands just have to “show up?”

    1. DNA isolation would be unsuccessful if no bands were shown in the image. The band length in DNA isolation does not matter it simply just needs to “show up.” In our future experiment when we would be running a restriction digest, the bands would matter of where the cuts in the DNA occurred.

  4. What exactly do we gain from the identification of the phage? Does confirmation that this mycobacteriophage is a siphoviridae have benefits in the bio- medical field?

    1. From identifying phage, we are able to cluster them into groups in order to be able to better determine which bacteria they are able to infect. Confirming whether the phage is siphoviridae or myoviridae is extremely important in the biomedical field because it allows us to cluster and identify the phages.

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