9 thoughts on “P68 – Roberts

  1. How would the use of UV light kill only the O cluster phage rather than both phages in the HTL?

    1. The study showed that some phage react differently to UV light so while some get killed off others may survive. We would need to conduct a multitude of experiments to determine if the O cluster phage can be killed off at a specific UV while the F cluster phage remains unaffected.

  2. As a cancer research student I am not familiar with any of this, that being said why does the clear plaques and diameter of 0.1 nanometers indicate that your phage is in the lytic cycle?

    1. Lytic phage will completely kill the host cell which is visualized with a clear plaque. Temperate phage will produce turbid plaques meaning they kill of some of the host but integrate into the host which keeps the bacteria from being killed off.

    2. Lytic phage kill off the host cell which visualized by a clear plaque. Temperate phage produce turbid plaques meaning they infect the cell by integrating into the host cell meaning some bacteria is killed off but they do not kill them off completely.

  3. You mention that in future experiments you would have to isolate the F cluster phage from the contaminate using UV light. Is there anything else that you could do if for whatever reason this doesn’t work?

    1. If UV does not work we can purify through many 1/10 dilutions of our lysate in hopes that we can isolate the F cluster phage from the O cluster phage.

    1. Each phage is different so conditions for one phage may cause them to lyse the cell while others remain integrated. But in general it may be a change in pH, environmental changes or damage to the bacterial cell.

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