Two of our initial enrichment samples suffered from L-Agar contamination. Moreover, one web pattern plate and one serial dilution suffered from an unknown source of contamination.
Two of our initial enrichment samples suffered from L-Agar contamination so we did have to collect new soil samples. Moreover, one web pattern plate and one serial dilution suffered from an unknown source of contamination.
Hi Hermann! Is there any way to predict whether your phage is lytic or lysogenic by looking at its microscopy image? Also how does a phage’s cluster determine its medical use/capabilities?
Electron Microscopy allows you to compare the ratio of the head in nm with that of the tail in nm, several establishes ratios exist which allow you to determine bacteriophage morphology. Our ratio happened to indicate siphoviridae. It also has a pretty pronounced tail length with respect to the tail head width so it is possible to predict the morphology without any ratio calculations as well. With regards to specific medical uses we can’t say anything for certain at the moment. However, Cluster A is one of the most well documented bacteriophages which means it’s more likely to have some sort of phage therapy application due to compatibility with certain bacteria/s.
What significance does “Wakandaforever” being in the A cluster have for its medical uses? I’m from outside the class, so I don’t really know how much this effects its morphology.
With regards to specific medical uses we can’t say anything for certain at the moment. However, Cluster A is one of the most well documented bacteriophages which means it’s more likely to have some sort of phage therapy application due to compatibility with certain bacteria/s.
Temperate phages commonly produce plaques which are halo like
and turbid whereas lytic phages form plaques which are clear. The use of temperate phages as therapeutics is problematic due to a combination of display of superinfection immunity which converts phage-sensitive bacteria into insensitive ones, and the encoding of bacterial virulence factors, including bacterial toxins. Therefore, bacteriophage therapy with lytic phages is better than temperate and the only means of currently antibacterial treatment.
Did you run into any setbacks during the semester?
Two of our initial enrichment samples suffered from L-Agar contamination. Moreover, one web pattern plate and one serial dilution suffered from an unknown source of contamination.
Do you think that the use of phage therapy in the medical field could become popularized one day?
I definitely think bacteriophage therapy will become the new norm for treating bacterial infections in say the next 10-20 years.
Did you have any issues while conducting any part of the experiment? Was this result off of your initial soil sample or did you have to collect more?
Two of our initial enrichment samples suffered from L-Agar contamination so we did have to collect new soil samples. Moreover, one web pattern plate and one serial dilution suffered from an unknown source of contamination.
Hi Hermann! Is there any way to predict whether your phage is lytic or lysogenic by looking at its microscopy image? Also how does a phage’s cluster determine its medical use/capabilities?
Electron Microscopy allows you to compare the ratio of the head in nm with that of the tail in nm, several establishes ratios exist which allow you to determine bacteriophage morphology. Our ratio happened to indicate siphoviridae. It also has a pretty pronounced tail length with respect to the tail head width so it is possible to predict the morphology without any ratio calculations as well. With regards to specific medical uses we can’t say anything for certain at the moment. However, Cluster A is one of the most well documented bacteriophages which means it’s more likely to have some sort of phage therapy application due to compatibility with certain bacteria/s.
What significance does “Wakandaforever” being in the A cluster have for its medical uses? I’m from outside the class, so I don’t really know how much this effects its morphology.
With regards to specific medical uses we can’t say anything for certain at the moment. However, Cluster A is one of the most well documented bacteriophages which means it’s more likely to have some sort of phage therapy application due to compatibility with certain bacteria/s.
What is the difference between a lytic or lysogenic phage; is one better for treatment in humans?
Temperate phages commonly produce plaques which are halo like
and turbid whereas lytic phages form plaques which are clear. The use of temperate phages as therapeutics is problematic due to a combination of display of superinfection immunity which converts phage-sensitive bacteria into insensitive ones, and the encoding of bacterial virulence factors, including bacterial toxins. Therefore, bacteriophage therapy with lytic phages is better than temperate and the only means of currently antibacterial treatment.