If our phage didn’t have turbid morphology, it could have had other types of morphologies such as a clear morphology. Different morphologies most likely meant our phage could have been lytic instead or still temperate.
You mentioned that your high titer lysate was contaminated, preventing you from moving on to other assays. What do you think was the source of contamination? Was this a common issue in the phage lab or was your group just unlucky?
I believe the source of our contamination could have been our web pattern plate or most dilute purification plate. This was not a common issue in the phage lab, and our group was just unlucky.
Overall, it was just due to the necessity to only have one species of phage over two. The larger turbid phage had the only plaques that properly purified and produce a consistency plaque morphology.
What would it mean if your phages hadn’t had turbid morphology ?
If our phage didn’t have turbid morphology, it could have had other types of morphologies such as a clear morphology. Different morphologies most likely meant our phage could have been lytic instead or still temperate.
If our phage didn’t have turbid morphology, it could have been a lytic phage instead or still a temperate phage with a slightly different morphology.
You presented your results and conclusions well. How were you able to purify your two species of phages into one?
We picked a plaque from both species of phage and performed a third purification until only one species presented a consistency plaque morphology.
You mentioned that your high titer lysate was contaminated, preventing you from moving on to other assays. What do you think was the source of contamination? Was this a common issue in the phage lab or was your group just unlucky?
I believe the source of our contamination could have been our web pattern plate or most dilute purification plate. This was not a common issue in the phage lab, and our group was just unlucky.
Great work! What made you choose to isolate one species of phage over the other?
Overall, it was just due to the necessity to only have one species of phage over two. The larger turbid phage had the only plaques that properly purified and produce a consistency plaque morphology.