17 thoughts on “P72 – Behnken

  1. That is really interesting, I’m sorry your bacteria got contaminated. What do you expect would have happened had the contamination not happened?

    1. Thank you! If we hadn’t seen such a wide variation in plaque sizes, we would have been able to archive our phage and send it off to Pittsburgh, where it would be frozen and potentially used for phage therapies.

  2. why do you think your electro-microscopy image did not reflect your previous results of contamination?

    1. We aren’t entirely sure! Since we took out such a small sample to perform electron microscopy, we could have just not picked up any contaminant particles, but this seems highly unlikely. We also filtered our flooded web pattern plate to create the high titer lysate, which could have filtered out the contaminant phage, but this is also unlikely, as this did not happen for other groups. Thanks for your question!

  3. I found your presentation really interesting! Despite knowing that you had a contaminant phage, your DNA analysis only showed there to be one strain. Why do you think that is?

    1. The DNA analysis was only for quality control, not for isolating a strain. Its purpose was to show if our DNA was good enough quality to perform future experiments.

  4. I really like the name of your phage! If you wanted to try to purify your phage again, would you be able to do that?

  5. I like the name of your phage! If you wanted to purify your phage of contamination would you be able to?

    1. Thank you! Unfortunately in our lab, we tried 6 times to purify our page, but it was unsuccessful. Given more time, we may be able, but it is uncertain.

    1. On each round of purification, there were two distinct plaque sizes: each 1 mm and 5 in size. These seemed to resemble two distinct species.

  6. In your future directions, you talk about exposing the phage with UV light to cause specific mutations that cause the phage to be dependent on a specific nutrient in the future. What kind of nutrients might be involved with these mutations?

  7. In your future directions, you mention triggering mutations that would cause the phage to require specific mutations using UV light. What kind of nutrients would be involved with this kind of mutation?

    1. Good question! It would depend on the type of mutation, but you could test which nutrients are required by testing on selective plates.

    2. The types of nutrients we would test would probably be similar to those in LB media, which we used to enrich our phage sample. This includes tryptone, yeast extract, and NaCl.

  8. How did you know that it was a siphoviridae morphology? What about the picture indicated that?

    1. We knew that our phage was siphoviridae due to its long tail length(on average, 138.39 nm)

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