In the future when you perform PCR again, what would you change about your procedure, if anything, and would you have different expectations for your results?
The problem with our PCR procedure was that our contents evaporated most likely during the denaturing stage due to the high temperatures. If I were to do this again, I would make sure that the lid is securley closed and I would check the temperature of the PCR machine to ensure it is not reaching higher temperatures than what is needed to denature the DNA. I would expect that our contents would not evaporate and we could successfuly amplified our target DNA.
Sure!! So a phage cocktail is when multiple species of phage that can affected the same bacteria are “mixed” together and used against the targeted bacteria in a phage therapy case. This decreases the chances of the targeted bacteria to becoming resistant of the phages and therefor uncurable.
Piggiwiggi was isolated from an enriched soil sample, so we did not nessicarily chose our phage, we instead isolated this specific species of phage. We named this species Piggiwiggi from a baby naming website!!
Yes I did, we ran into a contamination issue of our top agar and of our phage buffer for two of our experiments. We corrected this issue by getting new phage buffer and new top agar. Once these were attained we re-did the affected experiments. Thanks!!
This is a good question! The length of the body and structure of phage really just has to do with the morphology of the phage itself. But it also helps researches classify which family a phage can belong to. Phages can either be classified into Myoviridae, Podoviridae, Siphoviridae, or Inoviridae. Since our phage had a long tail and more or less rounded capsid or head, it is of the Siphoviridae family. Thanks so much!!
In the future when you perform PCR again, what would you change about your procedure, if anything, and would you have different expectations for your results?
The problem with our PCR procedure was that our contents evaporated most likely during the denaturing stage due to the high temperatures. If I were to do this again, I would make sure that the lid is securley closed and I would check the temperature of the PCR machine to ensure it is not reaching higher temperatures than what is needed to denature the DNA. I would expect that our contents would not evaporate and we could successfuly amplified our target DNA.
Could you embellish the application and composition of the phage cocktail?
Sure!! So a phage cocktail is when multiple species of phage that can affected the same bacteria are “mixed” together and used against the targeted bacteria in a phage therapy case. This decreases the chances of the targeted bacteria to becoming resistant of the phages and therefor uncurable.
What drove you to select this specific phage? Was there any specific reasoning behind choosing the Piggiwiggi phage?
Piggiwiggi was isolated from an enriched soil sample, so we did not nessicarily chose our phage, we instead isolated this specific species of phage. We named this species Piggiwiggi from a baby naming website!!
Did you have any difficulties during your research? If so how did they affect your project?
Great job!
Yes I did, we ran into a contamination issue of our top agar and of our phage buffer for two of our experiments. We corrected this issue by getting new phage buffer and new top agar. Once these were attained we re-did the affected experiments. Thanks!!
What does the length of the body and structure do for the function of the phage? How does this help with phage therapy?
Awesome job!
This is a good question! The length of the body and structure of phage really just has to do with the morphology of the phage itself. But it also helps researches classify which family a phage can belong to. Phages can either be classified into Myoviridae, Podoviridae, Siphoviridae, or Inoviridae. Since our phage had a long tail and more or less rounded capsid or head, it is of the Siphoviridae family. Thanks so much!!