12 thoughts on “P128 – Nagaraj

  1. What were experimental challenges that led you to isolate two phages instead of one? Thank you!

    1. I think that our main challenge was that the lysogenic plaques were so small and were often overcome by the lytic plaques because they were larger. I also think that there could have been some type of contamination that led to this or just my lab partner and I had a hard time recognizing different plaques.

  2. What were some experimental challenges that led you to isolate two phages instead of one?

    1. Our predictions were that we would be able to isolate a specific phage going into this from a sample of dirt, and this phage would infect M. Smeg.

  3. Can bacteria become phage resistant in a similar way to antibacterial resistance?

    1. Yes, it definitely can. This is due to the fact that bacteria can learn to recognize the phage and prevent the different phage from injecting the cell.

  4. Is isolating two phages problematic as far as determining specific phage clusters? Could these two phages be further isolated from each other in the future?

    1. Hello, This is an issue because the DNA we isolate is from both phages and since the DNA is all ran on the same gel with each other, there is no way for us to distinguish which pieces of DNA are from each phage. So, in order to isolate specific phage clusters of each phage, we would need to isolate each phage on its own to be able to run each of the different DNAs.

  5. Is the identification of specific clusters problematic considering that two phages were isolated? Can these two phages be further isolated?

  6. Hi great presentation! What makes this phage a good candidate for vaccine transport versus another similar type of phage?

    1. Thank you! The lysogenic phage is really good for transferring different vaccines, because it integrates its genome into the cell it is infecting. We can insert DNA or RNA needed from the vaccine into the phage, so then the phage will be able to integrate the vaccine nucleic acids into our cells, leading to them being able to recognize different diseases and make proteins to fight different diseases. However, this can still cause problems because the body can have a severe immune responses against phages due to the fact that it is still foreign to our bodies.

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