10 thoughts on “P130 – Reddy

  1. How does your results translate to the next step in testing? Does this then translate to animal studies or more lab studies?

    1. From the results gathered through this research, first we would need to complete more lab testing to be able to isolate the DNA, then proceed with PCR and restriction digest. This will let us know what cluster/sub cluster of bacteria our phage can infect. Once that information is gathered and with a more detailed plan of what bacteria can be killed, it could later on be used in animal studies to see how the phage reacts with the bacteria in an animal.

  2. What exactly is a phage? And how do you know which bacterias the phage can infect?

    1. A phage is a virus that can kill bacteria. Once the phages cluster can be identified, the bacteria that can be infected from this phage can be found due to knowing what clusters of phages can infect certain bacterias.

  3. I’m curious, when you mention that phages can typically kill things (I’m assuming unicellular organisms), what is it that they typically do kill?

    1. As stated in the background of the poster, phages have the capabilities to kill bacteria. This is important when seeing antibiotic resistant bacterias as phages can kill these kinds of bacterias.

  4. Great presentation!

    You mentioned your future directions would be to begin animal testing with this specific bacteriophage. What will this tell you after this research is performed? How will performing this next step effect the current knowledge we have on this bacteriophage?

    1. Since all the testing done for this experiment was done completely on plates, doing animal testing will tell us a lot on how this phage will react inside a body, starting with an animal. This will expand the current knowledge not just on this phage but in phages in general as there will be another phage identified that could kill an antibiotic resistant bacterial infection which in the long run helps advance phage therapy.

  5. How would you go about finding the kind of bacteria it would infect. Is there a criteria of bacteria this phage is more likely to infect?

    1. In order to find the types of bacteria that this phage would infect, further lab testing would need to be done. With where this experiment left off due to running out of time, we do not know what bacteria this phage is more likely to infect. In order to figure that out, the next steps would be to isolate the DNA, and perform PCR and restriction digest. This will give what cluster the phage is in to get a better understanding of what bacteria it can infect.

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