14 thoughts on “P78 – Kopp-Devol

  1. Is there a reason why phage therapy is useful to target? Why would we want to use phage therapy instead of traditional antibiotics?

    1. Traditional antibiotics are definitely the first choice for many reasons. Right now phage therapy is more of a last resort at least in human patients when they have infections of bacteria that are antibiotic resistant and other methods of treating the infection have failed.

  2. How confident are you in concluding that Rigby50 is temperate due to the contamination you found in your electron microscopy? Could this contamination have impacted this classification in any way?

    1. Good question! From what I know, the Corndog contaminate is lytic. So if the plaque morphology shows cloudy edges, it means that Rigby50 is indeed temperate, otherwise there would be only clear plaques.

  3. What benefit does phage therapy have over traditional antibiotics? I think you mentioned this being a possible alternative useful as antibiotic resistance becomes more widespread like it said in the poster, but was the implication that any alternative option might help or something else?

    Also, what made this phage specifically a possible treatment for tuberculosis as opposed to other infections?

    1. Antibiotics are the first choice for treatments, it is only when a bacterial infection is antibiotic resistant that phage therapy can be/needs to be used.
      As far as your second question, the phage that we isolated infects Mycobacterium smegmatis, which is a ‘cousin’ of Mycobacterium ttuberculosis. Because of this, they have similar binding sites that the phage can bind to and then infect.

      1. Great job on your research! I think you’re hitting on exactly what I’m curious about : Did you choose to investigate tuberculosis because the phage infects Mycobacterium smegmatis or did you set out to identify a new phage which could be useful in treating tuberculosis or similar diseases?

  4. In terms of your future directions, what do you expect to find in terms of more experiments with Rigby50?

    1. If we could, future directions would include isolating our phage to get rid of the contaminate Corndog. Along with this, redoing a restriction digest and also doing PCR would be important to help figure out the cluster more accurately.

  5. From Figure 3, what was the preliminary hypothesis regarding what cluster the phage was in?

    1. Our preliminary hypothesis was that our phage was in cluster A or O, and from further experiment results we narrowed it down to likely just the A cluster.

  6. So cool! Is there evidence or precedent for phages to be found just in “local” soil that have become useful? Have other phages been found in similar ways? Great work and great description!

    1. Phage are found everywhere that bacteria are found, which is everywhere! In fact, some phage have been isolated from navy ships (if I’m remembering correctly), and used as candidates for phage therapy. From this, I don’t think that the place that phage are found has much to do with the possibility for their ‘usefulness’. Hope this answers your question!

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