As we had to stop our lab at DNA isolation due to lack of time, we had only been able to determine that the phage was a myoviridae based on the EM images we obtained as well as the measurement that went along with. We were also able to calculate the titer which was 1.2×10^11 pfu/ml and also determined that the phage was a lytic one.
Yes it would allow to know which disease is compatible with our phage, since sequencing allows us to identify changes in genes and noncoding DNA, association with disease and identify the potential drug target.
Once we confirm the cluster, that would help us to determine exactly which types of phages we obtained, be it a new one or one that already exist. Then, if we had more time, we’d probably test the phage against other bacterial infections.
What is your expected result?
As we had to stop our lab at DNA isolation due to lack of time, we had only been able to determine that the phage was a myoviridae based on the EM images we obtained as well as the measurement that went along with. We were also able to calculate the titer which was 1.2×10^11 pfu/ml and also determined that the phage was a lytic one.
Use this link to access my presentation as a power point presentation. The video should play when you click start presentation.
file:///Users/vincentafetse/Downloads/P78-Afetse Flake.pptx
Do you think genomic sequencing would allow you to know which bacteria the phage could be used against as a therapy?
Yes it would allow to know which disease is compatible with our phage, since sequencing allows us to identify changes in genes and noncoding DNA, association with disease and identify the potential drug target.
This was a cool poster! What would be done once the cluster was confirmed for your phage?
Once we confirm the cluster, that would help us to determine exactly which types of phages we obtained, be it a new one or one that already exist. Then, if we had more time, we’d probably test the phage against other bacterial infections.