What indications did you see within your phage that gives the possibility that genetically modifying your phage means it would take on/ include a lytic cycle? Or is this something any phage that does not have a lytic cycle can undergo?
According to the figures shown from a plaque assay, it was determined that the species followed a temperate lifecycle because it had a clear center (display of lysing), and a halo around which demonstrated the lysogenic portion of the phage
You mentioned having good quality and quantity for one of your results what does that mean?
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For bands found on the gel electrophoresis they have good quality and quantity (good color and compacted)
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Did the soil from outside Porter being frozen alter this experiment in any way?
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The soil did not alter any outcomes of the enrichment process.
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Do the cluster groups N and A determine whether the phage is lytic/temperate? If so, how?
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The phage was determine as temperate throughout morphology notes in the experiments, the cluster groups helps identify similar phages.
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What indications did you see within your phage that gives the possibility that genetically modifying your phage means it would take on/ include a lytic cycle? Or is this something any phage that does not have a lytic cycle can undergo?
LikeLike
According to the figures shown from a plaque assay, it was determined that the species followed a temperate lifecycle because it had a clear center (display of lysing), and a halo around which demonstrated the lysogenic portion of the phage
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