16 thoughts on “P79 – Landry

  1. Hi! Thank you for your lovely presentation. I would love to hear more on what you feel is the most important future direction for this lab. And what do you think is the most feasible outcome of this future direction?

    1. Thank you for your commment! I feel as if the most important future direction would be to perform a PCR, because it would give us a more in-depth look at the isolated DNA allowing a better cluster hypothesis. With a better cluster hypothesis, we could determine if our phage could be used for phage therapy.

    1. Hi Dennis! An example of an unfavorable condition for a temperate phage could be a UV light. This type of stressor could allow the phage to exit the host genome and undergo the lytic lifecycle.

  2. This was a very nice and clear presentation, good job! I just was hoping to understand how EcoRI and KrabbiPatti being in the same cluster could lead to future discoveries and why it is significant that they are in the same cluster.

    1. Thank you Adi! The significance of EcoRI and KrabbiPatti being in the same cluster allows us to compare our isolated DNA to similar genomes and to have a better understanding of KrabbiPatti.

  3. Nice presentation! Are body size and shape the only thing that are used to determine the phage morphology (Siphoviridae)?

    1. Thank you Aidan! Body size and shape are not the only things that are used to determine phage morphology. When looking at our plaque assays, we can see that the plaques are small, clear, and concise which means it’s a lytic phage.

  4. I love the name of this fabulous little critter!
    What sorts of applications could KrabbiPatti have for bacterial research or other potential uses?

    1. Thank you! An application that KrabbiPatti could have is the participation in a “phage cocktail” ( a mix of different phages ) in order to be used in phage therapy.

  5. For your 2nd future direction, do you know what specific DNA sequence you would target? What goes into deciding what sequence to amplify to further characterize a phage’s cluster?

    1. Some things that go into performing a polymerase chain reaction is taking F1 and C1 primers (from our cluster hypothesis) and amplifying those sequences to recognize specific regions found within those clusters that could be in the DNA of KrabbiPatti.

    1. It all depends on the activity that takes place once a phage has entered its host cell. If the host cell bursts, then there is a lytic phage present. If there is replication taking place within the host cell, then it could be determined as a temperate phage but could enter a lytic life cycle depending on if there are environmental stressors present.

  6. Excellent job!
    Do you have any determinations on what might have caused the degradation of your CatDog sample?

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