8 thoughts on “P140 – Johnson

  1. Your poster is really interesting and well done. Was there a reason you choose to not include a DNA ladder in your Gel Electrophoresis Experiment?

    1. There was a miscommunication in which the DNA ladder was thought to be loaded, so it was a simple mistake that led to lack of a control within this experiment.

  2. You mentioned that your Titer could be considered relatively low, indicating a slow infection rate. What impact does this have on the desired outcome of the experiment? Does it impact the effectiveness of the phage?

    1. The impact that this had on the desired outcome of the experiment, is that we wanted a high titer in order to archive our phage into a national database, and we wanted a high rate of infection in order to kill off bacteria in a productive manner. It does impact the overall effectiveness of the phage as it causes a slower rate of infection meaning that less bacteria can be killed in a certain time interval.

  3. Wonderful articulation! Is there a reason why your lysate wasn’t of appropriate titer? What would this impact?

    1. There was not a specific reason that we concluded as to why the phage was a low titer, except for the fact that a HTL is calculated by the active phage particles per microliter, so there would be less active phage particles. The impact of this is it causes a slower rate of infection meaning that less bacteria will be lysed in a certain time interval allowing for continued growth of the bacterial colony.

  4. Well done, do you know what could have caused the lower quality DNA in the final gel?

    1. The best guess as to why the quality of the DNA was not as high was because something could have happened in the isolation process, however the quality was not as bad as the quantity which was much lower due to a lack of DNA being isolated.

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