6 thoughts on “P146 – Tran

    1. Hey there, yes I do! Unfortunately my video didn’t appear to upload until after 6 pm; I’m sorry about the delay. However, it is now accessible if you’re still looking to review the presentation — thank you!

  1. What about sequencing the phages genome do you think would be the most beneficial for researchers, possible proteins your phage has similar to other bacteriophage?

    1. I believe that sequencing a phage’s genome is most beneficial to researchers in allowing researchers to comprehensively compare the genome among other phage species — and discover how different mechanisms may be related to specific genome sequences. There is a lot we don’t know about phages as a whole, but categorizing them and identifying similarities in gene expression/protein synthesis/mechanisms allows us to find differences between phages. This allows us to narrow down the purpose or mechanism that each gene has on the phage and its gene expression — and what we might need to look for in order to increase the likelihood for phage infection in specific species, like M. tuberculosis!

  2. Great job! How much do you think the phage morphology/type affected your results? Or would affect their ability to treat antimicrobial resistant infections?

    1. Phage morphology (referring to its siphoviridae morphology that I touched upon) likely had many different effects on my results, but honestly I cannot be certain of its magnitude. Mycobacteriophages are often siphoviridae in its morphology, but I’m not sure how the restriction enzyme digest would have changed — for example — had the morphology been different. I’m not aware of how much the shape affects bacterial infection beyond its method/attachment process to enter the bacterium, but it is an interesting question to consider! I personally don’t think that the morphology should have a significant impact on the results obtained in my research — maybe except for the restriction enzyme digest results.

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